Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2

Y Wu, W Xu, Z Zhu, X Xia - Journal of Clinical Laboratory …, 2020 - Wiley Online Library
Background Reverse transcription‐polymerase chain reaction (RT‐PCR) is an extremely
common clinical method for detecting pathogens, particularly for emerging infectious …

rRT-PCR for SARS-CoV-2: Analytical considerations

R Rahbari, N Moradi, M Abdi - Clinica Chimica Acta, 2021 - Elsevier
The COVID-19 pandemic remains a significant problem involving health systems worldwide.
Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS …

[HTML][HTML] Comparison of seven commercial RT-PCR diagnostic kits for COVID-19

PB van Kasteren, B van Der Veer… - Journal of clinical …, 2020 - Elsevier
The final months of 2019 witnessed the emergence of a novel coronavirus in the human
population. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has since …

Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

U Eberle, C Wimmer, I Huber, A Neubauer-Juric… - European Journal of …, 2021 - Springer
To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the
detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating …

Molecular (real-time reverse transcription polymerase chain reaction) diagnosis of SARS-CoV-2 infections: complexity and challenges

S Sethi, T Chakraborty - Journal of Laboratory Medicine, 2021 - degruyter.com
The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) was first recorded in Wuhan, China. The World …

Analytic sensitivity of 3 nucleic acid detection assays in diagnosis of SARS-CoV-2 infection

JT Sieker, C Horowitz, CTK Hu… - The Journal of …, 2021 - academic.oup.com
Background Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
by reverse transcription PCR is the primary method to diagnose coronavirus disease 2019 …

Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories

T Ishige, S Murata, T Taniguchi, A Miyabe… - Clinica Chimica …, 2020 - Elsevier
Background The detection of SARS-CoV-2 RNA by real-time reverse transcription–
polymerase chain reaction (rRT-PCR) is used to confirm the clinical diagnosis of COVID-19 …

A novel multiplex qRT-PCR assay to detect SARS-CoV-2 infection: high sensitivity and increased testing capacity

S Petrillo, G Carrà, P Bottino, E Zanotto, MC De Santis… - Microorganisms, 2020 - mdpi.com
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is essential to limit the spread of the global pandemic we are facing. Quantitative real …

Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2

H Tombuloglu, H Sabit, E Al-Suhaimi, R Al Jindan… - Plos one, 2021 - journals.plos.org
The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV)
continues to increase globally. The real-time reverse transcription polymerase chain …

Comparing the analytical performance of three SARS-CoV-2 molecular diagnostic assays

K Uhteg, J Jarrett, M Richards, C Howard… - Journal of Clinical …, 2020 - Elsevier
In December 2019, a novel coronavirus (SARS-CoV-2) was first isolated from Wuhan city,
China and within three months, the global community was challenged with a devastating …