Luciferase enzymes from bioluminescent organisms can be expressed in mice, enabling
these rodents to glow when treated with a corresponding luciferin substrate. Light emission …

Bioluminescence imaging is a powerful approach for visualizing specific events occurring
inside live mice. Animals can be made to glow in response to the expression of a gene, the …

Firefly luciferase-catalyzed light emission from D-luciferin is widely used as a reporter of
gene expression and enzymatic activity both in vitro and in vivo. Despite the power of …

Luciferases have proven to be useful tools in advancing our understanding of biologic
processes. Having a multitude of bioluminescent reporters with different properties is highly …

Highlights•CycLuc1 outperforms D-luciferin for bioluminescence imaging in live
mice.•Luciferin substrates can be formed by reaction of component parts in vivo.•Caged …

In vivo bioluminescence imaging has become a cornerstone technology for preclinical
molecular imaging. This imaging method is based on light-emitting enzymes, luciferases …

Firefly luciferase adenylates and oxidizes d-luciferin to chemically generate visible light and
is widely used for biological assays and imaging. Here we show that both luciferase and …

Firefly bioluminescence is widely used in life science research as a useful analysis tool. For
example, the adenosine-5′-triphosphate (ATP)-dependent enzymatic firefly …

Bioluminescence imaging (BLI) is a widely applied visual approach for real-time detecting
many physiological and pathological processes in a variety of biological systems. Based on …

Bioluminescence imaging is a powerful, broadly utilized method for noninvasive imaging
studies in cell-based assays and small animal models of normal physiology and multiple …