Enzymes tend to malfunction when they work out of their natural cellular environments.
Engineering a favorable microenvironment around enzymes has emerged as an effective …

Immobilization depicts a propitious route to optimize the catalytic performances, efficient
recovery, minimizing autocatalysis, and also augment the stabilities of enzymes, particularly …

With the demand for green, safe, and continuous biocatalysis, bioscaffolds, compared with
synthetic scaffolds, have become a desirable candidate for constructing enzyme …

Enzymatic compartments, inspired by cell compartmentalization, which bring enzymes and
substrates together in confined environments, are of particular interest in ensuring the …

Immobilization of fragile enzymes in crystalline porous materials offers new opportunities to
expand the applications of biocatalysts. However, limited by the pore size and/or harsh …

Highlights•Cell-free enzyme systems offer advantages over whole-cells in chemical
biomanufacturing.•Multi-enzyme systems run step-wise reactions in one-pot with unique …

Background When using the microbial cell factories for green manufacturing, several
important issues need to be addressed such as how to maintain the stability of biocatalysts …

Significant advances in enzyme discovery, protein and reaction engineering have
transformed biocatalysis into a viable technology for the industrial scale manufacturing of …

The development of supramolecular chemistry has led to a shift in the research focus from
the structural design of supramolecules to developing functional systems, such as …

Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …