The effects of different fluorescent indicators in observing the changes of the mitochondrial membrane potential during oxidative stress-induced mitochondrial injury of …

Y Sun, K Zhou, M He, Y Gao, D Zhang, Y Bai… - Journal of …, 2020 - Springer
We evaluated the ability of different fluorescent indicators by various analytical instruments,
including a laser scanning confocal microscope (LSCM), fluorescence plate reader, and flow …

Imaging of reactive oxygen species burst from mitochondria using laser scanning confocal microscopy

J Xu, Z Hao, X Gou, W Tian, Y Jin, S Cui… - Microscopy …, 2013 - Wiley Online Library
Objective: Although several methods have been used to detect the intracellular reactive
oxygen species (ROS) generation, it is still difficult to determine where ROS generate from …

Dual-Emissive Probe for Reversible Visualization of ΔΨm Revealing Voltage Heterogeneity in a Single Mitochondrion

M Tian, J Sun, B Dong, Y Ma, W Lin - Analytical chemistry, 2021 - ACS Publications
Mitochondrial membrane potential (ΔΨm) is a fundamentally important parameter in
eukaryotic cells playing central roles in various vital biological processes. Precise …

[HTML][HTML] Mitochondrial mass assessment in a selected cell line under different metabolic conditions

A Costanzini, G Sgarbi, A Maresca, V Del Dotto… - Cells, 2019 - mdpi.com
Changes of quantity and/or morphology of cell mitochondria are often associated with
metabolic modulation, pathology, and apoptosis. Exogenous fluorescent probes used to …

[HTML][HTML] Isolated Mitochondria State after Myocardial Ischemia-Reperfusion Injury and Cardioprotection: Analysis by Flow Cytometry

C Crola Da Silva, D Baetz, M Védère, M Lo-Grasso… - Life, 2023 - mdpi.com
Rationale: Mitochondria are key organelles involved in cell survival and death during the
acute phenomena of myocardial ischemia-reperfusion (ie, myocardial infarction). To …

Detection of changes in mitochondrial function during apoptosis by simultaneous staining with multiple fluorescent dyes and correlated multiparameter flow cytometry

M Poot, RH Pierce - Cytometry: The Journal of the International …, 1999 - Wiley Online Library
Background: The possible relationships between changes in mitochondrial membrane
potential and other mitochondrial functions during apoptosis remain controversial. Methods …

Oxygen consumption rate to evaluate mitochondrial dysfunction and toxicity in cardiomyocytes

D Ahn, RE Go, KC Choi - Toxicological Research, 2023 - Springer
The increase in the types and complexity of diseases has led to significant advances in
diagnostic techniques and the availability of effective therapies. Recent studies have …

[HTML][HTML] Methods for mitochondrial health assessment by High Content Imaging System

C Panusatid, N Thangsiriskul, C Peerapittayamongkol - MethodsX, 2022 - Elsevier
Mitochondria are important organelles responsible for energy production. Mitochondrial
dysfunction relates to various pathological diseases. The investigation of mitochondrial …

Detection of mitochondrial depolarization/recovery during ischaemia–reperfusion using spectral properties of confocally recorded TMRM fluorescence

PW Venable, TG Taylor, KJ Sciuto… - The Journal of …, 2013 - Wiley Online Library
Key points• Mitochondrial inner membrane potential (ΔΨm) collapse during myocardial
ischaemia is one of the key events determining the physiological consequences of …

A flow cytometry-based assay for measuring mitochondrial membrane potential in cardiac myocytes after hypoxia/reoxygenation

G Chen, Y Yang, C Xu, S Gao - JoVE (Journal of Visualized Experiments), 2018 - jove.com
Timely and efficient reperfusion of the occluded coronary artery is the best strategy for
decreasing myocardial infarct size in patients with a ST-segment elevated myocardial …