The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite
for many standard experiments for the functional analysis of genes, including the generation …

The corn pathogen Ustilago maydis is a well-studied fungal model organism. Along with a
broad set of experimental tools, versatile strategies for the generation of gene replacement …

Methods to streamline functional studies of large numbers of genes are essential to fully
utilize the significant genomic resources now available for fungi. Fusion PCR is often used to …

Gene replacement via homologous double crossover in filamentous fungi requires relatively
long (preferentially> 0.5 kb) flanking regions of the target gene. For this reason, gene …

Model fungi such asNeurospora crassa, Aspergillus niger, andSaccharomyces
cerevisiaehave provided a wealth of genetic information and are currently the object of …

In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest
in the same strain (eg, to test for redundant gene function) is a difficult task due to the limited …

Current challenges in the study and biotechnological exploitation of filamentous fungi are
the optimization of DNA cloning and fungal genetic transformation beyond model fungi, the …

Advances in genetic transformation techniques have made important contributions to
molecular genetics. Various molecular tools and strategies have been developed for …

This article describes a split-marker approach, using two DNA fragments overlapping within
the dominant selectable marker in fungi. This approach has been shown to increase …

Genetic transformation methods are a prerequisite for the functional analysis and
modification of genes. About 30 years ago, first reports on the genetic transformation of …