In vitro assays: friends or foes of cell-penetrating peptides

J Liu, S Afshar - International Journal of Molecular Sciences, 2020 - mdpi.com
The cell membrane is a complex and highly regulated system that is composed of lipid
bilayer and proteins. One of the main functions of the cell membrane is the regulation of cell …

Studying the uptake of cell-penetrating peptides

T Holm, H Johansson, P Lundberg, M Pooga… - Nature protocols, 2006 - nature.com
More than a decade ago, it was discovered that cationic peptides could traverse the cellular
plasma membrane without specific transporter proteins or membrane damage …

Break on through to the other side—biophysics and cell biology shed light on cell‐penetrating peptides

R Fischer, M Fotin‐Mleczek, H Hufnagel… - …, 2005 - Wiley Online Library
Cell‐penetrating peptides (CPPs) have become widely used vectors for the cellular import of
molecules in basic and applied biomedical research. Despite the broad acceptance of these …

Challenge to overcome current limitations of cell-penetrating peptides

GC Kim, DH Cheon, Y Lee - Biochimica et Biophysica Acta (BBA)-Proteins …, 2021 - Elsevier
The penetration of biological membranes is a prime obstacle for the delivery of
pharmaceutical drugs. Cell-penetrating peptide (CPP) is an efficient vehicle that can deliver …

Cell-penetrating peptides: A comparative study on lipid affinity and cargo delivery properties

P Ruzza, B Biondi, A Marchiani, N Antolini, A Calderan - Pharmaceuticals, 2010 - mdpi.com
A growing number of natural and/or synthetic peptides with cell membrane penetrating
capability have been identified and described in the past years. These molecules have been …

Effects of Tryptophan Content and Backbone Spacing on Uptake Efficiency of Cell-Penetrating Peptides

HA Rydberg, M Matson, HL Åmand, EK Esbjörner… - Biophysical …, 2012 - cell.com
Cell-penetrating peptides (CPPs) have recently gained considerable interest due to their
ability to cross cellular membranes and deliver macromolecular cargo. It is of great …

On the importance of electrostatic interactions between cell penetrating peptides and membranes: a pathway toward tumor cell selectivity?

ML Jobin, ID Alves - Biochimie, 2014 - Elsevier
Cell-penetrating peptides (CPPs) are small molecules of major interest due to their ability to
efficiently transport cargos across cell membranes in a receptor-and energy-independent …

[PDF][PDF] Translocation of cell-penetrating peptides across biological membranes and interactions with plasma membrane constituents

J Pae - 2016 - core.ac.uk
Introduction of a vast number of biomolecules with high therapeutic potential into
experimental medical and clinical practice is hampered by the poor plasma membrane …

Applications and challenges for use of cell-penetrating peptides as delivery vectors for peptide and protein cargos

M Kristensen, D Birch, H Mørck Nielsen - International journal of …, 2016 - mdpi.com
The hydrophilic nature of peptides and proteins renders them impermeable to cell
membranes. Thus, in order to successfully deliver peptide and protein-based therapeutics …

[HTML][HTML] Assessing the uptake kinetics and internalization mechanisms of cell-penetrating peptides using a quenched fluorescence assay

I Mäger, E Eiríksdóttir, K Langel, SEL Andaloussi… - … et Biophysica Acta (BBA …, 2010 - Elsevier
Cell-penetrating peptides (CPPs) have shown great potency for cargo delivery both in vitro
and in vivo. Different biologically relevant molecules need to be delivered into appropriate …