Histological investigations are indispensable with regards to the identification of structural
tissue details but are limited to two-dimensional images, which are often visualized in one …

Many histological methods require staining of the cytoplasm, which provides instrumental
details for diagnosis. One major limitation is the production of 2D images obtained by …

Histology and immunohistochemistry of thin tissue sections have been the standard
diagnostic procedure in many diseases for decades. This method is highly specific for …

Background The University of Southampton, in collaboration with the University Hospital
Southampton (UHS) NHS Foundation Trust and industrial partners, has been at the forefront …

Despite the notable progress that has been made with nano-bio imaging probes,
quantitative nanoscale imaging of multistructured specimens such as mammalian cells …

Historically, micro-computed tomography (μCT) has been considered unsuitable for
histologic analysis of unstained formalin-fixed, paraffin-embedded soft tissue biopsy …

Examination of histological or immunohistochemically stained 2D sections of embedded
tissue is one of the most frequently used tools in biomedical research and clinical routine …

Although X-ray based 3D virtual histology is an emerging tool for the analysis of biological
tissue, it falls short in terms of specificity when compared to conventional histology. Thus, the …

The assessment of neuronal number, spatial organization and connectivity is fundamental
for a complete understanding of brain function. However, the evaluation of the three …

Deciphering the three-dimensional (3d) cytoarchitecture of neuronal tissue is an important
step towards understanding the connection between tissue function and structure and …