Moving human muscle physiology research forward: an evaluation of fiber type-specific protein research methodologies

IS Tobias, AJ Galpin - American Journal of Physiology-Cell …, 2020 - journals.physiology.org
Human skeletal muscle is a heterogeneous tissue composed of multiple fiber types that
express unique contractile and metabolic properties. While analysis of mixed fiber samples …

THRIFTY: a novel high‐throughput method for rapid fibre type identification of isolated skeletal muscle fibres

O Horwath, S Edman, A Andersson… - The Journal of …, 2022 - Wiley Online Library
Fibre type‐specific analyses are required for broader understanding of muscle physiology,
but such analyses are difficult to conduct due to the extreme time requirements of dissecting …

A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres

D Christiansen, MJ MacInnis, E Zacharewicz, H Xu… - Scientific reports, 2019 - nature.com
Many skeletal muscle proteins are present in a cell-specific or fibre-type dependent manner.
Stimuli such as exercise, aging, and disease have been reported to result in fibre-specific …

Protein profile of fiber types in human skeletal muscle: a single-fiber proteomics study

M Murgia, L Nogara, M Baraldo, C Reggiani, M Mann… - Skeletal muscle, 2021 - Springer
Background Human skeletal muscle is composed of three major fiber types, referred to as
type 1, 2A, and 2X fibers. This heterogeneous cellular composition complicates the …

Human skeletal muscle fiber type specific protein content

AJ Galpin, U Raue, B Jemiolo, TA Trappe… - Analytical …, 2012 - Elsevier
The aim of this project was to develop a method to assess fiber type specific protein content
across the continuum of human skeletal muscle fibers. Individual vastus lateralis muscle …

Fiber typing human skeletal muscle with fluorescent immunohistochemistry

KA Murach, CM Dungan, K Kosmac… - Journal of Applied …, 2019 - journals.physiology.org
Skeletal muscle myosin heavy chain (MyHC) fiber type composition is a critical determinant
of overall muscle function and health. Various approaches interrogate fiber type at the single …

Fiber-type traps: revisiting common misconceptions about skeletal muscle fiber types with application to motor control, biomechanics, physiology, and biology

SS Blemker, SV Brooks, KA Esser… - Journal of Applied …, 2024 - journals.physiology.org
Skeletal muscle is a highly complex tissue that is studied by scientists from a wide spectrum
of disciplines, including motor control, biomechanics, exercise science, physiology, cell …

Fiber type diversity in skeletal muscle explored by mass spectrometry-based single fiber proteomics

S Schiaffino, C Reggiani, M Murgia - 2020 - digitum.um.es
Mammalian skeletal muscles are composed of a variety of muscle fibers with specialized
functional properties. Slow fibers are suited for long lasting and low intensity contractile …

Transitions of muscle fiber phenotypic profiles

D Pette, RS Staron - Histochemistry and cell biology, 2001 - Springer
Skeletal muscle is a complex, versatile tissue composed of a large variety of functionally
diverse fiber types. The overall properties of a muscle largely result from a combination of …

A one-step immunostaining method to visualize rodent muscle fiber type within a single specimen

S Sawano, Y Komiya, R Ichitsubo, Y Ohkawa… - PLoS …, 2016 - journals.plos.org
In this study, we present a quadruple immunostaining method for rapid muscle fiber typing of
mice and rats using antibodies specific to the adult myosin heavy chain (MyHC) isoforms …