CRISPR/Cas9-based application for cancer therapy: challenges and solutions for non-viral delivery

YQ Lin, KK Feng, JY Lu, JQ Le, WL Li, BC Zhang… - Journal of Controlled …, 2023 - Elsevier
CRISPR/Cas9 genome editing is a promising therapeutic technique, which makes precise
and rapid gene editing technology possible on account of its high sensitivity and efficiency …

Challenges in CRISPR/CAS9 delivery: potential roles of nonviral vectors

L Li, ZY He, XW Wei, GP Gao, YQ Wei - Human gene therapy, 2015 - liebertpub.com
CRISPR/Cas9 genome editing platforms are widely applied as powerful tools in basic
research and potential therapeutics for genome regulation. The appropriate alternative of …

Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing

T Fang, X Cao, M Ibnat, G Chen - Journal of Nanobiotechnology, 2022 - Springer
The CRISPR-Cas9 technology has changed the landscape of genome editing and has
demonstrated extraordinary potential for treating otherwise incurable diseases. Engineering …

[HTML][HTML] Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicine

M Khoshandam, H Soltaninejad, M Mousazadeh… - Genes & …, 2024 - Elsevier
CRISPR/Cas9 is an effective gene editing tool with broad applications for the prevention or
treatment of numerous diseases. It depends on CRISPR (clustered regularly interspaced …

Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR–Cas9 plasmid

A Jo, VM Ringel-Scaia, DK McDaniel… - Journal of …, 2020 - Springer
Background The clustered regularly interspaced short palindromic repeats (CRISPR) and
Cas9 protein system is a revolutionary tool for gene therapy. Despite promising reports of …

Multistage delivery nanoparticle facilitates efficient CRISPR/dCas9 activation and tumor growth suppression in vivo

Q Liu, K Zhao, C Wang, Z Zhang, C Zheng… - Advanced …, 2019 - Wiley Online Library
CRISPR/dCas9 systems can precisely control endogenous gene expression without
interrupting host genomic sequence and have provided a novel and feasible strategy for the …

Progress and challenges towards CRISPR/Cas clinical translation

D Rosenblum, A Gutkin, N Dammes, D Peer - Advanced Drug Delivery …, 2020 - Elsevier
CRISPR/Cas systems (clustered regularly interspaced short palindromic repeats) have
emerged as powerful tools to manipulate the genome for both research and therapeutic …

Delivery systems of CRISPR/Cas9-based cancer gene therapy

A Biagioni, A Laurenzana, F Margheri, A Chillà… - Journal of Biological …, 2018 - Springer
Abstract CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is
today one of the most reliable method for gene-editing, supporting previous gene therapies …

Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide

H Kim, W Lee, Y Oh, SH Kang, JK Hur… - Nucleic acids …, 2020 - academic.oup.com
The CRISPR–Cas9 system is widely used for target-specific genome engineering. CRISPR–
Cas12a (Cpf1) is one of the CRISPR effectors that controls target genes by recognizing …

A single H1 promoter can drive both guide RNA and endonuclease expression in the CRISPR-Cas9 system

Z Gao, E Herrera-Carrillo, B Berkhout - Molecular Therapy-Nucleic Acids, 2019 - cell.com
The RNA-guided endonuclease Cas9 (CRISPR-Cas9) genome editing system has been
widely used for biomedical research and holds great potential for therapeutic applications in …