Agrobacterium tumefaciens-Mediated Transformation of NHEJ Mutant Aspergillus nidulans Conidia: An Efficient Tool for Targeted Gene Recombination Using …

V Casado-del Castillo, AP MacCabe, M Orejas - Journal of Fungi, 2021 - mdpi.com
Protoplast transformation for the introduction of recombinant DNA into Aspergillus nidulans
is technically demanding and dependant on the availability and batch variability of …

Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

E Kunitake, S Tani, J Sumitani, T Kawaguchi - Amb Express, 2011 - Springer
Agrobacterium tumefaciens-mediated transformation (AMT) was applied to Aspergillus
aculeatus. Transformants carrying the T-DNA from a binary vector pBIG2RHPH2 were …

The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection …

SE Unkles, EI Campbell… - Molecular and General …, 1989 - Springer
The development of an efficient and homologous transformation system for Aspergillus
oryzae is described. This is based on nitrate reductase (nia D) of the nitrate assimilation …

Efficient gene targeting in Penicillium chrysogenum using novel Agrobacterium-mediated transformation approaches

P de Boer, J Bronkhof, K Dukiќ, R Kerkman… - Fungal Genetics and …, 2013 - Elsevier
The industrial production of β-lactam antibiotics by Penicillium chrysogenum has increased
tremendously over the last decades, however, further optimization via classical strain and …

Improved Transformation of the Filamentous Fungus Aspergillus niger Using Agrobacterium tumefaciens

SM Park - Mycobiology, 2001 - Taylor & Francis
Since it is known that Agrobacterium tumefaciens, which has long been used to transform
plants, can transfer the T-DNA to yeast Saccharomyces cerevisiae during tumourigenesis, a …

Agrobacterium tumefaciens-Mediated Transformation

RJN Frandsen - Genetic Transformation Systems in Fungi, Volume 1, 2015 - Springer
The use of Agrobacterium tumefaciens-mediated transformation for achieving genetic
transformation of fungi has steadily increased over the last decade, and has proven to be …

[HTML][HTML] Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through …

C Ropero-Pérez, P Manzanares, JF Marcos… - Current Research in …, 2024 - Elsevier
In the last years, many research efforts have been applied for the development of
filamentous fungi as hosts for heterologous protein production. Aspergillus vadensis CBS …

A novel improved method for Aspergillus nidulans transformation

M Koukaki, E Giannoutsou, A Karagouni… - Journal of microbiological …, 2003 - Elsevier
We systematically investigated the efficiency of Aspergillus nidulans transformation using
protoplasts prepared from different stages of conidiospore germination and young mycelium …

Transformation of Aspergillus awamori by Agrobacterium tumefaciens–mediated homologous recombination

RJ Gouka, C Gerk, PJJ Hooykaas, P Bundock… - Nature …, 1999 - nature.com
Agrobacterium tumefaciens is known to transfer part of its tumor-inducing (Ti) plasmid to the
filamentous fungus Aspergillus awamori by illegitimate recombination with the fungal …

Agrobacterium-mediated transformation of the filamentous fungus Aspergillus awamori

CB Michielse, PJ J Hooykaas… - Nature protocols, 2008 - nature.com
Many transformation methods have been developed to introduce DNA into filamentous
fungi. One of these methods is Agrobacterium-mediated transformation (AMT). Here, we …