Analysis of external quality assessment samples revealed crucial performance differences between commercial RT-PCR assays for SARS-CoV-2 detection when …

M Malecki, J Luesebrink, AF Wendel… - Journal of Virological …, 2021 - Elsevier
In limelight of the ongoing pandemic SARS-CoV-2 testing is critical for the diagnosis of
infected patients, contact-tracing and mitigating the transmission. Diagnostic laboratories are …

Analytical sensitivity comparison of 14 conventional and three rapid RT-PCR assays for SARS-CoV-2 detection

X Wang, Z Huang, J Song, Y Xiao, H Wang - Journal of Virological Methods, 2021 - Elsevier
Recent reports have compared the analytical sensitivities of some SARS-CoV-2 RT-PCR
assays, but differences in the viral materials used for these evaluations made …

Potential pitfalls in analysing a SARS-CoV-2 RT-PCR assay and how to standardise data interpretation

M Smith, K El Bouzidi, S Bengen, A Cohen… - Journal of Virological …, 2022 - Elsevier
The emergence of SARS-CoV-2 in December 2019 lead to the rapid implementation of
assays for virus detection, with real-time RT-PCR arguably considered the gold-standard. In …

[HTML][HTML] Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

JJ LeBlanc, JB Gubbay, Y Li, R Needle… - Journal of Clinical …, 2020 - Elsevier
With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential.
This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV …

Critical aspects concerning the development of a pooling approach for SARS-CoV-2 diagnosis using large-scale PCR testing

D Cruceriu, O Baldasici, L Balacescu, S Gligor-Popa… - Viruses, 2021 - mdpi.com
The primary approach to controlling the spread of the pandemic SARS-CoV-2 is to diagnose
and isolate the infected people quickly. Our paper aimed to investigate the efficiency and the …

Analytical performance of COVID-19 detection methods (RT-PCR): scientific and societal concerns

R Verna, W Alallon, M Murakami, CPM Hayward… - Life, 2021 - mdpi.com
Background. Health and social management of the SARS-CoV-2 epidemic, responsible for
the COVID-19 disease, requires both screening tools and diagnostic procedures. Reliable …

Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2

Y Wu, W Xu, Z Zhu, X Xia - Journal of Clinical Laboratory …, 2020 - Wiley Online Library
Background Reverse transcription‐polymerase chain reaction (RT‐PCR) is an extremely
common clinical method for detecting pathogens, particularly for emerging infectious …

[HTML][HTML] Evaluation of extraction-free RT-PCR methods for faster and cheaper detection of SARS-CoV-2 using two commercial systems

F Morecchiato, M Coppi, I Baccani, N Maggini… - International Journal of …, 2021 - Elsevier
Objective When using high-throughput batched diagnostic platforms based on RT-PCR for
SARS-CoV-2 detection, avoidance of the conventional nucleic acid extraction step can help …

Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements

B Visseaux, G Collin, N Houhou-Fidouh… - Journal of Virological …, 2021 - Elsevier
The worldwide demand for SARS-CoV-2 RT-PCR testing resulted in a shortage of
diagnostic kits. RNA extraction step constitutes a major bottleneck to perform diagnostic. The …

Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constrained settings

MS Muller, SB Chhetri, C Basham, T Rapp… - … and Infectious Disease, 2021 - Elsevier
Alternatives to nasopharyngeal sampling are needed to increase capacity for SARS-CoV-2
testing. Among 275 participants, we piloted the collection of nasal mid-turbinate swabs …