[HTML][HTML] A comparative study of real-time RT-PCR–based SARS-CoV-2 detection methods and its application to human-derived and surface swabbed material

A Tastanova, CI Stoffel, A Dzung, PF Cheng… - The Journal of Molecular …, 2021 - Elsevier
Real-time RT-PCR remains a gold standard in the detection of various viral diseases. In the
coronavirus 2019 pandemic, multiple RT-PCR–based tests were developed to screen for …

Evaluation of intra-and interlaboratory variations in SARS-CoV-2 real-time RT-PCR through nationwide proficiency testing

K Park, H Sung, S Chun, WK Min - Laboratory Medicine, 2022 - academic.oup.com
Objective This study aimed to examine the intra-and interlaboratory variations of cycle
threshold (Ct) values using the nationwide proficiency testing for SARS-CoV-2. Methods …

Diagnostic accuracy of three commercially available one step RT-PCR assays for the detection of SARS-CoV-2 in resource limited settings

A Sisay, A Abera, B Dufera, T Endrias, G Tasew… - PloS one, 2022 - journals.plos.org
Background COVID-19 is an ongoing public health pandemic regardless of the countless
efforts made by various actors. Quality diagnostic tests are important for early detection and …

[HTML][HTML] Analytical and clinical performances of seven direct detection assays for SARS-CoV-2

Y Matsumura, W Yamazaki, T Noguchi… - Journal of Clinical …, 2023 - Elsevier
Background Direct detection tests for severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) that bypass complicated nucleic acid/antigen purification steps are promising …

Reliability of real-time RT-PCR tests to detect SARS-Cov-2: A literature review

C Bezier, G Anthoine, A Charki - International Journal of …, 2020 - metrology-journal.org
In the face of the COVID-19 (Coronavirus Disease 2019) pandemic, the World Health
Organization (WHO) has urged countries to test the population more widely. Clinical …

Analytic sensitivity of 3 nucleic acid detection assays in diagnosis of SARS-CoV-2 infection

JT Sieker, C Horowitz, CTK Hu… - The Journal of …, 2021 - academic.oup.com
Background Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
by reverse transcription PCR is the primary method to diagnose coronavirus disease 2019 …

Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic

E Krause, J Michel, A Puyskens, N Hofmann, T Rinner… - Virology Journal, 2023 - Springer
Background Over the course of the COVID-19 pandemic, laboratories worldwide have been
facing an unprecedented increase in demand for PCR testing because of the high …

Nationwide harmonization effort for semi-quantitative reporting of SARS-CoV-2 PCR test results in Belgium

L Cuypers, J Bode, K Beuselinck, L Laenen… - Viruses, 2022 - mdpi.com
From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing
indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to …

Lab practices that improve coronavirus disease 2019 detection accuracy using real-time PCR

G Soufan, N Berro, S Nahle, M Darwish… - JBI Evidence …, 2022 - journals.lww.com
The number of coronavirus disease 2019 (COVID-19) cases significantly increased with the
emergence of multiple variants of severe acute respiratory syndrome coronavirus-2 (SARS …

rRT-PCR for SARS-CoV-2: Analytical considerations

R Rahbari, N Moradi, M Abdi - Clinica Chimica Acta, 2021 - Elsevier
The COVID-19 pandemic remains a significant problem involving health systems worldwide.
Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS …