Electron paramagnetic resonance (EPR) is a spectroscopic method for investigating
structures, conformational changes, and dynamics of biomacromolecules, for example …

Due to the conformational variability of riboswitches, their structural analysis is of a great
importance. The most frequently used techniques to investigate dynamic processes within …

Understanding the function of oligonucleotides on a molecular level requires methods for
studying their structure, conformational changes, and internal dynamics. Various biophysical …

RNAs are important biomolecules that play essential roles in various cellular processes and
are crucially linked with many human diseases. The key to elucidate the mechanisms …

Ribonucleic acid function is governed by its structure, dynamics, and interaction with other
biomolecules and influenced by the local environment. Thus, methods are needed that …

Site-directed spin labeling is a powerful tool for investigating the conformation and dynamics
of biomacromolecules such as RNA. Here we introduce a spin labeling strategy based on …

Non-covalent and site-directed spin labeling gives easy access to spin-labeled nucleic acids
for the study of their structure and dynamics by electron paramagnetic resonance (EPR) …

To gain information about the function of nucleic acids, their structure and dynamics need to
be investigated. Electron paramagnetic resonance (EPR)-and fluorescence spectroscopies …

The site-specific introduction of spin labels into RNA for distance measurements by EPR
gives insight into its solution structure. We here present a method for spin labeling of in vitro …

In this protocol, we describe the facile synthesis of the nitroxide spin-label 2, 2, 5, 5-
tetramethyl-pyrrolin-1-oxyl-3-acetylene (TPA) and then its coupling to DNA/RNA through …