Simple and efficient chemically defined in vitro maturation and embryo culture system for bovine embryos

ME Arias, T Vargas, V Gallardo, L Aguila, R Felmer - Animals, 2022 - mdpi.com
Simple Summary Supplementation of the culture media for the in vitro production of bovine
embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. This study …

A single medium supports development of bovine embryos throughout maturation, fertilization and culture

AP Gandhi, M Lane, DK Gardner… - Human …, 2000 - academic.oup.com
Oocytes and embryos are typically exposed sequentially to varying culture media in
standard in-vitro protocols. Expenditures of energy may be required following each medium …

Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media

BD Bavister, TA Rose-Hellekant, T Pinyopummintr - Theriogenology, 1992 - Elsevier
The techniques of in vitro maturation (IVM) and in vitro fertilization (IVF) of bovine oocytes,
with development of the resulting embryos to advanced preimplantation stages in vitro, have …

A serum-free medium for use in a cumulus cell co-culture system for bovine embryos derived from in vitro maturation and in vitro fertilization

JM Lim, A Rocha, W Hansel - Theriogenology, 1996 - Elsevier
The objective of this study was to develop a serum-free medium for the co-culture of bovine
embryos that would yield a percentage of blastocysts equal to that obtained with fetal bovine …

Developmental potential of bovine oocytes cultured in different maturation and culture conditions

H Sagirkaya, M Misirlioglu, A Kaya, NL First… - Animal Reproduction …, 2007 - Elsevier
Diverse groups of chemicals in culture media are needed for successful bovine oocyte
maturation and embryo development during which dramatic cytoplasmic and nuclear …

The differential requirement of albumin and sodium citrate on the development of in vitro produced bovine embryos

LY Sung, F Du, J Xu, W Chang… - Reproduction …, 2004 - rnd.edpsciences.org
In vitro culture for bovine embryos is largely not optimal. Our study was to determine the
components necessary for early embryo development. In experiment 1, IVF embryos were …

[PDF][PDF] Kinetics of early in vitro development of bovine in vivo-and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

P Holm, PJ Booth, H Callesen - REPRODUCTION-CAMBRIDGE-, 2002 - academia.edu
It is generally accepted that bovine embryos produced in vitro have a lower developmental
capacity than do embryos produced in vivo. Large field trials under commercial conditions …

[HTML][HTML] Development and quality of bovine embryos produced in vitro using growth factor supplemented serum-free system

A Dhali, VM Anchamparuthy, SP Butler… - Open Journal of Animal …, 2011 - scirp.org
The influence of a growth factor supplemented serum-free system on the development, gene
expression, and cryotolerance of in vitro pro-duced bovine embryos was investigated. To …

Pregnancy rates after transfer of fresh or frozen bovine blastocysts developed from serum-free or protein-free media

ES Lee, Y Okamoto, H Yamashina, Y Fukui - Theriogenology, 1997 - infona.pl
There are few data available on in vivo viability of bovine embryos developed from serum-
free or protein-free medium without somatic cell coculture. The objective of this study was to …

Establishment of an advanced chemically defined medium for early embryos derived from in vitro matured and fertilized bovine oocytes

K MOMOzAwA, Y FuKuDA - Journal of Reproduction and …, 2011 - jstage.jst.go.jp
A chemically defined medium would be useful for analyzing promoters or inhibitors in in vitro
culture (IVC) of bovine embryos. However, an IVC system for bovine embryos in a …