The functioning of bioluminescent systems in most of the known marine organisms is based
on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by …

Recent advances in bioluminescence technology rely on the discovery of luciferin-luciferase
pairs having excellent luminescence activity. Here, we focus on coelenterazine (CTZ) and its …

Bioluminescence is a widespread natural phenomenon. Luminous organisms are found
among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies …

A unique combinatorial bioluminescence (BL) imaging system was developed for
determining molecular events in mammalian cells with various colors and BL intensity …

Bioluminescence-based techniques, such as bioluminescence imaging, BRET and dual-
luciferase reporter assay systems, have been widely used to examine a myriad of biological …

The cold-induced expression system in Escherichia coli is useful and we have applied this
system to prepare the coelenterazine-utilizing luciferases including Renilla luciferase …

It has been shown that the coelenterazine analog, coelenterazine-v, is an efficient substrate
for a reaction catalyzed by Renilla luciferase. The resulting bioluminescence emission …

In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes,
luciferases, and delivery of chemical substrates to expressing cells. Coelenterazine (CLZN) …

Ca2+-triggered coelenterazine-binding protein (CBP) is a natural form of the luciferase
substrate involved in the Renilla bioluminescence reaction. It is a stable complex of …

The recombinant coelenterazine-dependent luciferases (isoforms MLuc164 and MLuc39)
from the marine copepod Metridia longa were expressed asinclusion bodies in E. coli cells …