This review article gathers the available information on the use of embryo-tests as high-
throughput tools for toxicity screening, hazard assessment and prioritization of new and …

Background, aim, and scope The use of fish embryos is not regulated by current legislations
on animal welfare and is therefore considered as a refinement, if not replacement of animal …

Addressing the shift from classical animal testing to high-throughput in vitro and/or simplified
in vivo proxy models has been defined as one of the upcoming challenges in aquatic …

The validation of high-throughput toxicity tests with invertebrate species is a key priority to
improve hazard assessment of new chemicals and increase the available test guidelines …

The zebrafish embryo toxicity test has been proposed as an alternative for the acute fish
toxicity test, which is required by various regulations for environmental risk assessment of …

Animal alternative tests are gaining serious consideration in an array of environmental
sciences, particuarly as they relate to sound management of chemicals and wastewater …

The main available alternatives for testing embryotoxicity are cellular tests with stem cells
and in vitro-ex vivo tests with embryos. In cellular tests, the most developed alternative is the …

The embryonic zebrafish is a powerful tool for high-throughput screening of chemicals.
While this model has significant potential for use in safety assessments and chemical …

Typically, time‐consuming standard toxicological assays using the zebrafish (Danio rerio)
embryo model evaluate mortality and teratogenicity after exposure during the first 2 days …

The movement away from mammalian testing of potential toxicants and new chemical
entities has primarily led to cell line testing and protein-based assays. However, these …