This chapter focuses on bioluminescent imaging in vivo and how the development of
modern luciferase systems enable this goal. A diverse set of organisms naturally emit light …

Current mammalian bioreporters using either firefly luciferase (luc) or GFP constructs require
lysis and/or exogenous excitation to evoke a measurable response. Consequently, these …

Bioluminescence imaging of single cells is often complicated by the requirement of
exogenous luciferins that can be poorly cell-permeable or produce high background signal …

Bioluminescent and fluorescent reporter systems have enabled the rapid and continued
growth of the optical imaging field over the last two decades. Of particular interest has been …

<? xm-replace_text {Heading}?> Abstract Bioluminescence imaging (BLI) of bacteria was
primarily designed to permit real-time, sensitive, and noninvasive monitoring of the …

Bioluminescence-based imaging of living cells has become an important tool in biological
and medical research. However, many bioluminescence imaging applications are limited by …

The bacterial luciferase gene cassette (lux) is an ideal bioreporter for real-time monitoring of
the dynamics of bacteria because it is a fully autonomous, substrate-free bioluminescent …

Bioluminescent production represents a facile method for bioreporter detection in
mammalian tissues. The lack of endogenous bioluminescent reactions in these tissues …

The lux operon is a useful reporter for bioluminescence imaging due to its independence of
exogenous luciferin supply, but its relatively low brightness hampers the imaging of single …

In the deep, dark ocean, many living organisms orchestrate one of the most amazing light
shows the eye can ever witness. Obscured by total darkness and surrounded by predators …