CRISPR/Cpf1-mediated mutagenesis and gene deletion in industrial filamentous fungi Aspergillus oryzae and Aspergillus sojae
T Katayama, J Maruyama - Journal of bioscience and bioengineering, 2022 - Elsevier
In industrial applications such as fermentation and heterologous protein production, various
Aspergillus oryzae and A. sojae strains are used. Although genetic engineering techniques …
Aspergillus oryzae and A. sojae strains are used. Although genetic engineering techniques …
Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae
T Katayama, Y Tanaka, T Okabe, H Nakamura… - Biotechnology …, 2016 - Springer
Objectives To develop a genome editing method using the CRISPR/Cas9 system in
Aspergillus oryzae, the industrial filamentous fungus used in Japanese traditional …
Aspergillus oryzae, the industrial filamentous fungus used in Japanese traditional …
Development of a CRISPR/Cpf1 system for multiplex gene editing in Aspergillus oryzae
T Chen, Z Chen, H Zhang, Y Li, L Yao, B Zeng… - Folia Microbiologica, 2024 - Springer
CRISPR/Cas technology is a powerful tool for genome engineering in Aspergillus oryzae as
an industrially important filamentous fungus. Previous study has reported the application of …
an industrially important filamentous fungus. Previous study has reported the application of …
CRISPR/Cas9-Based Genome Editing and Its Application in Aspergillus Species
FJ Jin, BT Wang, ZD Wang, L Jin, P Han - Journal of Fungi, 2022 - mdpi.com
Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …
crucial roles in the decomposition of organic materials as an important environmental …
CRISPR/Cas9-Mediated Multiplexed Genome Editing in Aspergillus oryzae
Aspergillus oryzae has great potential and competitive advantages to be developed as an
excellent expression system, owing to its powerful protein secretion ability, complex post …
excellent expression system, owing to its powerful protein secretion ability, complex post …
Efficient genome editing in filamentous fungi via an improved CRISPR‐Cas9 ribonucleoprotein method facilitated by chemical reagents
G Zou, M Xiao, S Chai, Z Zhu, Y Wang… - Microbial …, 2021 - Wiley Online Library
DNA double‐strand break (DSB) repair induced by the RNA‐programmed nuclease Cas9
has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR …
has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR …
A CRISPR/Cas9-mediated gene knockout system in Aspergillus luchuensis mut. kawachii
C Kadooka, M Yamaguchi, K Okutsu… - Bioscience …, 2020 - academic.oup.com
We developed an approach to genome editing of the white koji fungus, Aspergillus
luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats …
luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats …
Genome Editing Technology and Its Application Potentials in the Industrial Filamentous Fungus Aspergillus oryzae
J Maruyama - Journal of Fungi, 2021 - mdpi.com
Aspergillus oryzae is a filamentous fungus that has been used in traditional Japanese
brewing industries, such as the sake, soy sauce, and miso production. In addition, A. oryzae …
brewing industries, such as the sake, soy sauce, and miso production. In addition, A. oryzae …
Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems
Y Zhang, L Ouyang, Y Nan, J Chu - Bioresources and Bioprocessing, 2019 - Springer
Aspergillus niger, as an important industrial strain, is widely used in the production of a
variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger …
variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger …
CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …
production. With the increasing number of full genomes available for fungal species, the …