In industrial applications such as fermentation and heterologous protein production, various
Aspergillus oryzae and A. sojae strains are used. Although genetic engineering techniques …

Objectives To develop a genome editing method using the CRISPR/Cas9 system in
Aspergillus oryzae, the industrial filamentous fungus used in Japanese traditional …

CRISPR/Cas technology is a powerful tool for genome engineering in Aspergillus oryzae as
an industrially important filamentous fungus. Previous study has reported the application of …

Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …

Aspergillus oryzae has great potential and competitive advantages to be developed as an
excellent expression system, owing to its powerful protein secretion ability, complex post …

DNA double‐strand break (DSB) repair induced by the RNA‐programmed nuclease Cas9
has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR …

Aspergillus niger is a vital industrial workhouse widely used for the production of organic
acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance …

We developed an approach to genome editing of the white koji fungus, Aspergillus
luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats …

Aspergillus oryzae is a filamentous fungus that has been used in traditional Japanese
brewing industries, such as the sake, soy sauce, and miso production. In addition, A. oryzae …

Aspergillus niger, as an important industrial strain, is widely used in the production of a
variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger …