Background The University of Southampton, in collaboration with the University Hospital
Southampton (UHS) NHS Foundation Trust and industrial partners, has been at the forefront …

Historically, micro-computed tomography (μCT) has been considered unsuitable for
histologic analysis of unstained formalin-fixed, paraffin-embedded soft tissue biopsy …

Histology and immunohistochemistry of thin tissue sections have been the standard
diagnostic procedure in many diseases for decades. This method is highly specific for …

Histological investigations are indispensable with regards to the identification of structural
tissue details but are limited to two-dimensional images, which are often visualized in one …

Abstract 3D imaging approaches based on X-ray microcomputed tomography (microCT)
have become increasingly accessible with advancements in methods, instruments and …

For over a hundred years, the histological study of tissues has been the gold standard for
medical diagnosis because histology allows all cell types in every tissue to be identified and …

Whereas the two-dimensional (2D) visualisation of biological samples is routine, three-
dimensional (3D) imaging remains a time-consuming and relatively specialised pursuit …

Three-dimensional (3D) analysis is an essential tool to obtain quantitative results from 3D
datasets. Considerable progress has been made in 3D imaging techniques, resulting in a …

Laboratory transmission soft X-ray microscopy (L-TXM) has emerged as a complementary
tool to synchrotron-based TXM and high-resolution biomedical 3D imaging in general in …

The basic requirements of microscopical imaging for biological specimens–contrast among
tissues and resolvability of histological features–present persistent challenges for whole …