Upland cotton (Gossypium hirsutum), an allotetraploid, contains At-and Dt-subgenome and
most genes have multiple homologous copies, which pose a huge challenge to investigate …

Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system is emerging as effective strategy for generating site-specific …

As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

Plant virus-mediated sgRNA delivery and expression have great advantages; sgRNA
expression can rapidly expand and accumulate along with virus replication and movement …

The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …

Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …

Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …

Genome editing has been a long-term challenge for molecular biology research, particularly
for plants possess complex genome. The recently discovered Clustered Regularly …

Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …