gRNA validation for wheat genome editing with the CRISPR-Cas9 system

T Arndell, N Sharma, P Langridge, U Baumann… - BMC …, 2019 - Springer
Abstract Background The CRISPR-Cas9 system is a powerful and versatile tool for crop
genome editing. However, achieving highly efficient and specific editing in polyploid species …

CRISPR/Cas system: recent advances and future prospects for genome editing

H Manghwar, K Lindsey, X Zhang, S Jin - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …

High‐throughput genome editing in rice with a virus‐based surrogate system

Y Tian, D Zhong, X Li, R Shen, H Han… - Journal of Integrative …, 2023 - Wiley Online Library
With the widespread use of clustered regularly interspaced palindromic repeats
(CRISPR)/CRISPR‐associated nuclease (Cas) technologies in plants, large‐scale genome …

Genome editing technologies and their applications in crop improvement

R Mishra, K Zhao - Plant Biotechnology Reports, 2018 - Springer
Crop improvement is very essential to meet the increasing global food demands and
enhance food nutrition. Conventional crop-breeding methods have certain limitations such …

CRISPR/Cas9-Based Genome Editing Toolbox for Arabidopsis thaliana

D Miki, G Zinta, W Zhang, F Peng, Z Feng, JK Zhu - Arabidopsis Protocols, 2021 - Springer
CRISPR/Cas9 system has emerged as a powerful genome engineering tool to study gene
function and improve plant traits. Genome editing is achieved at a specific genome …

[HTML][HTML] Efficient multiplex genome editing by CRISPR/Cas9 in common wheat

J Li, S Zhang, R Zhang, J Gao, Y Qi… - Plant biotechnology …, 2021 - ncbi.nlm.nih.gov
Efficient multiplex genome editing by CRISPR/Cas9 in common wheat - PMC Back to Top Skip
to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI Homepage Main …

Optimizing multiplex CRISPR/Cas9-based genome editing for wheat

W Wang, A Akhunova, S Chao, E Akhunov - BioRxiv, 2016 - biorxiv.org
Background CRISPR/Cas9-based genome editing holds great promise to accelerate the
development of improved crop varieties by providing a powerful tool to modify the genomic …

CRISPR/Cas9: an RNA‐guided highly precise synthetic tool for plant genome editing

Y Demirci, B Zhang, T Unver - Journal of cellular physiology, 2018 - Wiley Online Library
CRISPR/Cas9 is a newly developed and naturally occurred genome editing tool, which is
originally used by bacteria for immune defence. In the past years, it has been quickly …

Efficiency, specificity and temperature sensitivity of Cas9 and Cas12a RNPs for DNA-free genome editing in plants

R Banakar, M Schubert, G Kurgan, KM Rai… - Frontiers in Genome …, 2022 - frontiersin.org
Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs)
transfection offers several advantages over plasmid DNA-based delivery methods, including …

Expanding the range of CRISPR/Cas9 genome editing in rice

X Hu, C Wang, Y Fu, Q Liu, X Jiao, K Wang - Molecular plant, 2016 - cell.com
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …