gRNA validation for wheat genome editing with the CRISPR-Cas9 system
T Arndell, N Sharma, P Langridge, U Baumann… - BMC …, 2019 - Springer
Abstract Background The CRISPR-Cas9 system is a powerful and versatile tool for crop
genome editing. However, achieving highly efficient and specific editing in polyploid species …
genome editing. However, achieving highly efficient and specific editing in polyploid species …
CRISPR/Cas system: recent advances and future prospects for genome editing
H Manghwar, K Lindsey, X Zhang, S Jin - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
High‐throughput genome editing in rice with a virus‐based surrogate system
Y Tian, D Zhong, X Li, R Shen, H Han… - Journal of Integrative …, 2023 - Wiley Online Library
With the widespread use of clustered regularly interspaced palindromic repeats
(CRISPR)/CRISPR‐associated nuclease (Cas) technologies in plants, large‐scale genome …
(CRISPR)/CRISPR‐associated nuclease (Cas) technologies in plants, large‐scale genome …
Genome editing technologies and their applications in crop improvement
R Mishra, K Zhao - Plant Biotechnology Reports, 2018 - Springer
Crop improvement is very essential to meet the increasing global food demands and
enhance food nutrition. Conventional crop-breeding methods have certain limitations such …
enhance food nutrition. Conventional crop-breeding methods have certain limitations such …
CRISPR/Cas9-Based Genome Editing Toolbox for Arabidopsis thaliana
CRISPR/Cas9 system has emerged as a powerful genome engineering tool to study gene
function and improve plant traits. Genome editing is achieved at a specific genome …
function and improve plant traits. Genome editing is achieved at a specific genome …
[HTML][HTML] Efficient multiplex genome editing by CRISPR/Cas9 in common wheat
J Li, S Zhang, R Zhang, J Gao, Y Qi… - Plant biotechnology …, 2021 - ncbi.nlm.nih.gov
Efficient multiplex genome editing by CRISPR/Cas9 in common wheat - PMC Back to Top Skip
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Optimizing multiplex CRISPR/Cas9-based genome editing for wheat
Background CRISPR/Cas9-based genome editing holds great promise to accelerate the
development of improved crop varieties by providing a powerful tool to modify the genomic …
development of improved crop varieties by providing a powerful tool to modify the genomic …
CRISPR/Cas9: an RNA‐guided highly precise synthetic tool for plant genome editing
CRISPR/Cas9 is a newly developed and naturally occurred genome editing tool, which is
originally used by bacteria for immune defence. In the past years, it has been quickly …
originally used by bacteria for immune defence. In the past years, it has been quickly …
Efficiency, specificity and temperature sensitivity of Cas9 and Cas12a RNPs for DNA-free genome editing in plants
Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs)
transfection offers several advantages over plasmid DNA-based delivery methods, including …
transfection offers several advantages over plasmid DNA-based delivery methods, including …
Expanding the range of CRISPR/Cas9 genome editing in rice
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …