Background CRISPR-Cas genome editing technologies have revolutionized
biotechnological research particularly in functional genomics and synthetic biology. As an …

Abstracts CRISPR/Cas technology is a versatile tool for genome engineering in many
organisms, including filamentous fungi. Cpf1 is a multi-domain protein of class 2 (type V) …

Filamentous fungi represent an invaluable source of pharmaceutically active compounds.
The development of versatile methods to genetically manipulate filamentous fungi is of great …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

In recent years, a variety of genetic tools have been developed and applied to various
filamentous fungi, which are widely applied in agriculture and the food industry. However …

The CRISPR/Cas9 system is a revolutionary genome editing tool. However, in eukaryotes,
search and optimization of a suitable promoter for guide RNA expression is a significant …

Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …

CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome
editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools …

Abstract Background The CRISPR/Cas9 is currently the predominant technology to enhance
the genome editing efficiency in eukaryotes. Established tools for many fungal species exist …

CRISPR/Cas technology is a powerful tool for genome engineering in Aspergillus oryzae as
an industrially important filamentous fungus. Previous study has reported the application of …