In a biological system, the spatiotemporal arrangement of enzymes in a dense cellular
milieu, subcellular compartments, membrane-associated enzyme complexes on cell …

Co-immobilization of multi-enzymes has emerged as a promising concept to design and
signify bio-catalysis engineering. Undoubtedly, the existence and importance of basic …

Immobilized enzymes as biocatalysts have great potential both scientifically and industrially
because of their technological and economic importance. Their highly efficient catalytic …

In living cells enzymes catalyze a wide variety of metabolic processes, which involve
multiple reaction steps. Efficient transfer of the intermediates from one catalytic site to the …

Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …

Enzymes have received extensive research due to their high selectivity, efficiency, and
environmental protection. However, the lack of stability and reusability drastically hinders the …

Nature relies on complexes of colocated enzymes to efficiently perform multiple catalytic
steps. Such enzyme colocalisation promotes substrate channelling, enhances the activity of …

Multi-enzyme biocatalysis is an important technology to produce many valuable chemicals
in the industry. Different strategies for the construction of multi-enzyme systems have been …

Highlights•Cell-free enzyme systems offer advantages over whole-cells in chemical
biomanufacturing.•Multi-enzyme systems run step-wise reactions in one-pot with unique …

Multienzymatic cascade reactions are a most important technology to succeed in industrial
process development, such as synthesis of pharmaceutical, cosmetic, and nutritional …