Coupling chemical and biological catalysis: a flexible paradigm for producing biobased chemicals

TJ Schwartz, BH Shanks, JA Dumesic - Current opinion in biotechnology, 2016 - Elsevier
Highlights•Produce biobased chemicals using both chemical and biological catalysis.•Use
biological catalysis to convert sugars to de-functionalized platform molecules.•Upgrade …

Immobilization of enzymes on nanoinorganic support materials: An update

Z Ashkan, R Hemmati, A Homaei, A Dinari… - International Journal of …, 2021 - Elsevier
Despite the widespread use in various industries, enzyme's instability and non-reusability
limit their applications which can be overcome by immobilization. The nature of the enzyme's …

In situ Immobilization of Multi-Enzymes for Enhanced Substrate Channeling of Enzyme Cascade Reactions: A Nanoarchitectonics Approach by Directed Metal …

D Fernando, M Mathesh, J Cai, W Yang - Langmuir, 2023 - ACS Publications
Rationally tailoring a controlled spatial organization of enzymes in a nanoarchitecture for
multi-enzyme cascade reactions can enhance the catalytic efficiency via substrate …

Multienzymatic nanoassemblies: recent progress and applications

A Giannakopoulou, E Gkantzou, A Polydera… - Trends in …, 2020 - cell.com
Biotechnological research has turned to multienzymatic nanoassemblies as a promising
concept to host multiple applications. Here, we consider important aspects around the …

Tools and strategies for constructing cell-free enzyme pathways

K Petroll, D Kopp, A Care, PL Bergquist… - Biotechnology advances, 2019 - Elsevier
Single enzyme systems or engineered microbial hosts have been used for decades but the
notion of assembling multiple enzymes into cell-free synthetic pathways is a relatively new …

New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

RA Sheldon, A Basso, D Brady - Chemical Society Reviews, 2021 - pubs.rsc.org
This tutorial review focuses on recent advances in technologies for enzyme immobilisation,
enabling their cost-effective use in the bio-based economy and continuous processing in …

Immobilized biocatalysts: Novel approaches and tools for binding enzymes to supports

P Torres‐Salas, A del Monte‐Martinez, B Cutiño‐Avila… - 2011 - Wiley Online Library
In the last decade, new trends for enzyme attachment to solid carriers have emerged in an
attempt to rationalize the classical methods for enzyme immobilization. In silico analysis is …

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Biotechnology …, 2015 - Elsevier
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

Mapping out the degree of freedom of hosted enzymes in confined spatial environments

Q Sun, Y Pan, X Wang, H Li, J Farmakes, B Aguila… - Chem, 2019 - cell.com
The integration of enzymes with solid materials is crucial for promoting their industrialization.
Understanding the enzyme behavior upon association within a confined space, though of …

Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks

G Chen, S Huang, X Ma, R He, G Ouyang - Nature Protocols, 2023 - nature.com
Enzymes are outstanding natural catalysts with exquisite 3D structures, initiating countless
life-sustaining biotransformations in living systems. The flexible structure of an enzyme …