Substrates used to culture human embryonic stem cells (hESCs) are typically 2-dimensional
(2-D) in nature, with limited ability to recapitulate in vivo-like 3-dimensional (3-D) …

We report optimization of a serum-and feeder-free, three-dimensional (3D) niche created
with a synthetic polyethylene glycol (PEG)-based extracellular matrix for self-renewal of …

Human embryonic stem cells (hESCs) have the ability to proliferate indefinitely and
differentiate into each of the embryonic cell lineages. Great care is required to maintain …

Recently emerging evidence has indicated surface nanotopography as an important
physical parameter in the stem cell niche for regulating cell fate and behaviors for various …

Human embryonic stem (hES) cells are generally cultured as cell clusters on top of a feeder
layer formed by mitotically inactivated murine embryonic fibroblasts (MEFs) to maintain their …

We have developed a synthetic polymer interface for the long-term self-renewal of human
embryonic stem cells (hESCs) in defined media. We successfully cultured hESCs on …

Inadequate cell numbers in culture is one of the hurdles currently delaying the application of
human embryonic stem cells (hESCs) for transplantation therapy. Nanofibrous scaffolds …

Stem cell-based tissue engineering is a promising technology in the effort to create
functional tissues of choice. To establish an efficient approach for generating hematopoietic …

The potential of embryonic stem (ES) cells for both self-renewal and differentiation into cells
of all three germ layers has generated immense interest in utilizing these cells for tissue …

The ability of embryonic stem (ES) cells to self-renew indefinitely and to differentiate into
multiple cell lineages holds promise for advances in modeling disease progression …