The current status and trends of DNA extraction

X Ye, B Lei - Bioessays, 2023 - Wiley Online Library
DNA extraction, playing an irreplaceable role in molecular biology as it is an essential step
prior to various downstream biological analyses. Thus, the accuracy and reliability of …

[PDF][PDF] The evolution of DNA extraction methods

M Dairawan, PJ Shetty - Am. J. Biomed. Sci. Res, 2020 - pdfs.semanticscholar.org
Since the first DNA extraction performed by Friedrich Miescher in 1869, scientists have
made extraordinary progress in designing extraction methods that are more reliable, easier …

DNA extraction: finding the most suitable method

C Barbosa, S Nogueira, M Gadanho… - Molecular microbial …, 2016 - Elsevier
Nowadays, detection of food and water pathogens is an issue of major concern. DNA-based
methods are simple, sensitive, specific, and able to detect low amounts of the target, making …

[HTML][HTML] Development of an innovative and sustainable one-step method for rapid plant DNA isolation for targeted PCR using magnetic ionic liquids

A Marengo, C Cagliero, B Sgorbini, JL Anderson… - Plant Methods, 2019 - Springer
Background Nowadays, there is an increasing demand for fast and reliable plant
biomolecular analyses. Conventional methods for the isolation of nucleic acids are time …

Extraction of DNA by magnetic ionic liquids: tunable solvents for rapid and selective DNA analysis

KD Clark, O Nacham, H Yu, T Li, MM Yamsek… - Analytical …, 2015 - ACS Publications
DNA extraction represents a significant bottleneck in nucleic acid analysis. In this study,
hydrophobic magnetic ionic liquids (MILs) were synthesized and employed as solvents for …

[HTML][HTML] SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species

S Vilanova, D Alonso, P Gramazio, M Plazas… - Plant Methods, 2020 - Springer
Background The use of sequencing and genotyping platforms has undergone dramatic
improvements, enabling the generation of a wealth of genomic information. Despite this …

Direct extraction of genomic DNA from maize with aqueous ionic liquid buffer systems for applications in genetically modified organisms analysis

E Gonzalez García, AK Ressmann, P Gaertner… - Analytical and …, 2014 - Springer
To date, the extraction of genomic DNA is considered a bottleneck in the process of
genetically modified organisms (GMOs) detection. Conventional DNA isolation methods are …

Modified low‐salt CTAB extraction of high‐quality DNA from contaminant‐rich tissues

JR Arseneau, R Steeves… - Molecular ecology …, 2017 - Wiley Online Library
The increasing use of high‐throughput sequencing platforms has made the isolation of pure,
high molecular weight DNA a primary concern for studies of a diverse range of organisms …

[HTML][HTML] Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids

MN Emaus, C Cagliero, MR Gostel, G Johnson… - Plant Methods, 2022 - Springer
Background Plant DNA isolation and purification is a time-consuming and laborious process
relative to epithelial and viral DNA sample preparation due to the cell wall. The lysis of plant …

Arabidopsis thaliana ITS sequence-specific DNA extraction by ion-tagged oligonucleotides coupled with a magnetic ionic liquid

A Marengo, MN Emaus, CM Bertea, C Bicchi… - Analytical and …, 2019 - Springer
This study reports a follow-up investigation on the capture of specific DNA sequences using
ion-tagged oligonucleotides (ITOs) and magnetic ionic liquids (MIL). Five allylimidazolium …