Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative

Y Zhou, F Pei, M Ji, L Wang, H Zhao, H Li, W Yang… - PloS one, 2020 - journals.plos.org
The early detection and differential diagnosis of respiratory infections increase the chances
for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the …

[HTML][HTML] Clinical performance and analytical sensitivity of three SARS-CoV-2 nucleic acid diagnostic tests

B Freire-Paspuel… - The American journal of …, 2021 - ncbi.nlm.nih.gov
Hundreds of RT-qPCR kits are available in the market for SARS-CoV-2 diagnosis, some of
them with emergency use authorization (EUA) by the Food Drug Administration (FDA) or …

SARS-CoV-2 detection using digital PCR for COVID-19 diagnosis, treatment monitoring and criteria for discharge

R Lu, J Wang, M Li, Y Wang, J Dong, W Cai - MedRxiv, 2020 - medrxiv.org
Background SARS-CoV-2 nucleic acid detection by RT-PCR is one of the criteria approved
by China FDA for diagnosis of COVID-19. However, inaccurate test results (for example …

An updated review of SARS‐CoV‐2 detection methods in the context of a novel coronavirus pandemic

Y Zhang, Z Huang, J Zhu, C Li, Z Fang… - Bioengineering & …, 2023 - Wiley Online Library
Abstract The World Health Organization has reported approximately 430 million confirmed
cases of coronavirus disease 2019 (COVID‐19), caused by severe acute respiratory …

Rapid detection of SARS-CoV-2 using reverse transcription RT-LAMP method

W Yang, X Dang, Q Wang, M Xu, Q Zhao, Y Zhou… - MedRxiv, 2020 - medrxiv.org
Abstract Corona Virus Disease 2019 (COVID-19) is a recently emerged life-threatening
disease caused by SARS-CoV-2. Real-time fluorescent PCR (RT-PCR) is the clinical …

Comparison and sensitivity evaluation of three different commercial real-time quantitative PCR kits for SARS-CoV-2 detection

A Banko, G Petrovic, D Miljanovic, A Loncar… - Viruses, 2021 - mdpi.com
Real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most sensitive
and specific assay and, therefore, is the “gold standard” diagnostic method for the diagnosis …

Analysis of the initial lot of the CDC 2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel

JS Lee, JM Goldstein, JL Moon, O Herzegh… - PLoS …, 2021 - journals.plos.org
At the start of the COVID-19 pandemic, the Centers for Disease Control and Prevention
(CDC) designed, manufactured, and distributed the CDC 2019-Novel Coronavirus (2019 …

Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2

H Tombuloglu, H Sabit, E Al-Suhaimi, R Al Jindan… - Plos one, 2021 - journals.plos.org
The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV)
continues to increase globally. The real-time reverse transcription polymerase chain …

Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2

YJ Jung, GS Park, JH Moon, K Ku, SH Beak, S Kim… - BioRxiv, 2020 - biorxiv.org
Abstract Coronavirus disease 2019 (COVID-19) is newly emerging human infectious
diseases, which is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS …

Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR

M Park, J Won, BY Choi, CJ Lee - Experimental & molecular medicine, 2020 - nature.com
SARS-CoV-2 is very contagious and has rapidly spread globally. Due to various
symptomatic and asymptomatic cases and the possibility of asymptomatic transmission …