Non-adherent cells are difficult to transfect with chemical-mediated delivery methods.
Electroporation is an attractive strategy to transfer the molecules of interest into suspension …

Gene electrotransfer (GET) is a widely used method for nucleic acids' delivery into cells. We
explored, evaluated, and demonstrated the potential use of different pulse durations for …

We introduced eukaryotic expression plasmid pEGFP-N1 encoding green fluorescent
protein (GFP) genes into cells with different biological features through electroporation. The …

Abstract Knowledge of the parameters which influence the efficiency of gene electrotransfer
has importance for practical implementation of electrotransfection for gene therapy as well …

Gene electrotransfer is an effective and promising gene delivery technique in clinical
applications, such as DNA vaccination and gene therapy. An improved gene therapy …

Electroporation as a delivery method is increasingly important in gene therapy, not only in
vivo but also in in vitro experimental systems. Different applications of gene electrotransfer …

Availability of an efficient transfection protocol is the first determinant in success of gene
transferring studies in mammalian cells which is accomplished experimentally for every …

Physical methods are widely utilized to deliver nucleic acids into cells such as electro-
transfection or heat shock. An efficient gene electro-transfection requires the best conditions …

Background Gene electrotransfer is an established method that enables transfer of DNA into
cells with electric pulses. Several studies analyzed and optimized different parameters of …

Electroporation is an electro-physical, non-viral approach to perform DNA, RNA, and protein
transfections of cells. Upon application of an electric field, the cell membrane is …