Several patients with severe combined immunodeficiency-X1 disease and adenosine
deaminase deficiency have been cured by retroviral-mediated gene therapy. Despite the …

The use of retroviral vectors for human gene therapy requires the production of large
quantities of high titer vector stocks. Maintaining high titers during the prolonged culture of …

Retroviruses are currently the most widely used vectors in clinical trials for gene therapy.
These vectors are, however, limited by low titres partly due to the restrictive nature of …

The development of gene therapy is hampered by the difficulty of producing large stocks of
retroviral vectors at high titer. This study aimed to improve culture conditions and to intensify …

Retroviral vectors are still the vectors that are used in the majority of gene therapy trials for
treatment of acquired or inherited diseases. In this review, the present state‐of‐the‐art of the …

Background The use of gene modified T lymphocytes for immunotherapy in a cancer or
AIDS clinical trial requires an efficient, safe ex vivo method for modification of these cells at …

For centuries mankind led a difficult battle against viruses, the smallest infectious agents at
the surface of the earth. Nowadays it is possible to use viruses for our benefit, both at a …

Retroviral vectors are presently amongst the most widely used vectors in gene therapy
clinical trials to target pathologies of different origins, such as cancers, genetic diseases or …

Background For many inherited and acquired diseases of the blood system, gene transfer
into hematopoietic cells is a promising strategy to alleviate disease‐related symptoms or …

Retroviral infection or calcium phosphate-mediated DNA transfection has been used for the
generation of retrovirus producing cell lines through the introduction of vector DNA into the …