Improved, human‐based packaging cell lines allow the production of high‐titer, RCR‐free
retroviral vectors. The utility of these cell lines for the production of clinical grade vectors …

Retroviral vectors derived from murine leukemia virus (MLV) are used in somatic gene
therapy applications eg for genetic modification of hematopoietic stem cells. Recently, we …

The potential for gene therapy to cure a wide range of diseases has lead to high
expectations and a great increase in research efforts in this area. At present, viral vectors are …

Abstract Background Retroviral vectors (RVs) constitute one of the preferred gene therapy
tools against inherited and acquired diseases. Development of scaleable downstream …

Retroviral vectors are powerful tools for gene transfer that are useful in the context of
experimental as well as clinical applications. Defective recombinant retroviruses allow for …

The production and stability of recombinant retroviral vectors was examined at various
temperatures. The two studied recombinant retroviral vectors, based on different packaging …

A combination of adenoviral and retroviral vectors was used to construct second generation
packaging cells that deliver marker genes to target cells. A vector based upon Moloney …

Transient transfection of 293T cells was utilized to produce high-titer murine recombinant
retroviral vectors for clinical studies. This system was initially optimized by gene transfer …

Obtaining high expression levels of a therapeutic gene in target cells could be achieved by
integrating multiple copies of a recombinant retrovirus. However, we observed that cells …

Many current gene therapy protocols use retroviruses as the vector to package and transfer
genes to target cells. Cocultivation of target cells on a layer of irradiated retrovirus producing …