[PDF][PDF] Permeabilized cryopreserved human hepatocytes as an exogenous metabolic system in a novel
H Wei, AP Li - researchgate.net
We report here a novel in vitro experimental system, the metabolism-dependent cytotoxicity
assay (MDCA), for the definition of the roles of hepatic drug metabolism in toxicity. MDCA …
assay (MDCA), for the definition of the roles of hepatic drug metabolism in toxicity. MDCA …
Permeabilized cryopreserved human hepatocytes as an exogenous metabolic system in a novel metabolism-dependent cytotoxicity assay for the evaluation of …
H Wei, AP Li - Drug Metabolism and Disposition, 2022 - ASPET
We report here a novel in vitro experimental system, the metabolism-dependent cytotoxicity
assay (MDCA), for the definition of the roles of hepatic drug metabolism in toxicity. MDCA …
assay (MDCA), for the definition of the roles of hepatic drug metabolism in toxicity. MDCA …
A novel in vitro experimental system for the evaluation of drug metabolism: cofactor-supplemented permeabilized cryopreserved human hepatocytes (MetMax …
We report here a novel experimental system, cryopreserved MetMax human hepatocytes
(MMHHs), for in vitro drug metabolism studies. MMHHs consist of cofactor-supplemented …
(MMHHs), for in vitro drug metabolism studies. MMHHs consist of cofactor-supplemented …
Cryopreserved human hepatocytes: characterization of drug-metabolizing activities and applications in higher throughput screening assays for hepatotoxicity …
AP Li, C Lu, JA Brent, C Pham, A Fackett… - Chemico-biological …, 1999 - Elsevier
Cryopreserved human hepatocytes were extensively characterized in our laboratory. The
post-thaw viability, as determined by Trypan blue exclusion, ranged from, the viability …
post-thaw viability, as determined by Trypan blue exclusion, ranged from, the viability …
Evaluation of Adverse Drug Properties with Cryopreserved Human Hepatocytes and the Integrated Discrete Multiple Organ Co-culture (IdMOCTM) System
AP Li - Toxicological research, 2015 - Springer
Human hepatocytes, with complete hepatic metabolizing enzymes, transporters and
cofactors, represent the gold standard for in vitro evaluation of drug metabolism, drug-drug …
cofactors, represent the gold standard for in vitro evaluation of drug metabolism, drug-drug …
Long-term stability of cryopreserved human hepatocytes: Evaluation of phase I and II drug-metabolizing enzyme activities and CYP3A4/5 induction for more than a …
M Sudo, M Nishihara, J Takahashi, S Asahi - Drug Metabolism and …, 2017 - ASPET
We evaluated the long-term stability of hepatocytes stored in the vapor phase of liquid
nitrogen for their viability, cytochrome P450 (CYP) 1A2 activity, CYP3A4/5 activity, uridine …
nitrogen for their viability, cytochrome P450 (CYP) 1A2 activity, CYP3A4/5 activity, uridine …
Development and validation of a higher-throughput cytochrome P450 inhibition assay with the novel cofactor-supplemented permeabilized cryopreserved human …
VRC Palacharla, P Chunduru, DR Ajjala… - Drug Metabolism and …, 2019 - ASPET
Here, we report the application of a novel hepatocyte system, the cofactor-supplemented
permeabilized cryopreserved human hepatocytes [MetMax human hepatocytes (MMHHs)] in …
permeabilized cryopreserved human hepatocytes [MetMax human hepatocytes (MMHHs)] in …
[PDF][PDF] Validation of Pooled Cryopreserved Human Hepatocytes as a Model for Metabolism Studies
JG Zhang, CM Chandler, A Dandeneau, CL Crespi… - Drugs - researchgate.net
Primary human hepatocytes are an important model system for xenobiotic metabolism and
toxicity studies. However, the demand for fresh human hepatocytes far exceeds current …
toxicity studies. However, the demand for fresh human hepatocytes far exceeds current …
A high-throughput cell-based toxicity analysis of drug metabolites using flow cytometry
EJ Buenz - Cell Biology and Toxicology, 2007 - Springer
The effects of liver enzymes on drug activities are important considerations in the drug
discovery process. Frequently, liver microsomes are used to simulate first-pass metabolism …
discovery process. Frequently, liver microsomes are used to simulate first-pass metabolism …
Human hepatocytes as a tool for studying toxicity and drug metabolism
MJ Gomez-Lechon, MT Donato… - Current drug …, 2003 - ingentaconnect.com
Drugs are usually biotransformed into new chemical species that may have either toxic or
therapeutic effects. Drug metabolism studies are routinely performed in laboratory animals …
therapeutic effects. Drug metabolism studies are routinely performed in laboratory animals …