rRT-PCR for SARS-CoV-2: Analytical considerations

R Rahbari, N Moradi, M Abdi - Clinica Chimica Acta, 2021 - Elsevier
The COVID-19 pandemic remains a significant problem involving health systems worldwide.
Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS …

[HTML][HTML] Comparison of seven commercial RT-PCR diagnostic kits for COVID-19

PB van Kasteren, B van Der Veer… - Journal of clinical …, 2020 - Elsevier
The final months of 2019 witnessed the emergence of a novel coronavirus in the human
population. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has since …

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

JF Huggett, V Benes, SA Bustin, JA Garson… - Clinical …, 2020 - academic.oup.com
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the worldwide
struggle against COVID-19, is capable of detecting a single molecule of a viral genome …

Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19

M Teymouri, S Mollazadeh, H Mortazavi… - … -Research and Practice, 2021 - Elsevier
Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-
CoV-2), the control of virus spread has remained challenging given the pitfalls of the current …

[HTML][HTML] Detection of SARS-CoV-2 infection by RT-PCR test: factors influencing interpretation of results

K Munne, V Bhanothu, V Bhor, V Patel, SD Mahale… - Virusdisease, 2021 - Springer
In this current pandemic of coronavirus disease 2019 (COVID-19), prompt interventions in
terms of early detection and clinical management along with isolation of positive cases is of …

[HTML][HTML] Real-time RT-PCR in COVID-19 detection: issues affecting the results

A Tahamtan, A Ardebili - Expert review of molecular diagnostics, 2020 - Taylor & Francis
Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19)
cases caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 …

[HTML][HTML] Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection

J Alcoba-Florez, H Gil-Campesino… - International Journal of …, 2020 - Elsevier
Objectives The ongoing COVID-19 pandemic continues to impose demands on diagnostic
screening. In anticipation that the recurrence of outbreaks and the measures for lifting the …

Comparison of COVID‐19 laboratory diagnosis by commercial kits: Effectivity of RT‐PCR to the RT‐LAMP

Y Artik, AB Coşğun, NP Cesur, N Hızel… - Journal of medical …, 2022 - Wiley Online Library
Coronavirus disease 2019 or COVID‐19 caused by novel coronavirus/severe acute
respiratory syndrome coronavirus 2 (SARS‐CoV‐2 or 2019‐nCoV) is an ongoing pandemic …

[HTML][HTML] Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)

G Lippi, AM Simundic, M Plebani - Clinical Chemistry and Laboratory …, 2020 - degruyter.com
A novel zoonotic coronavirus outbreak is spreading all over the world. This pandemic
disease has now been defined as novel coronavirus disease 2019 (COVID-19), and is …

[HTML][HTML] A novel multiplex qRT-PCR assay to detect SARS-CoV-2 infection: high sensitivity and increased testing capacity

S Petrillo, G Carrà, P Bottino, E Zanotto, MC De Santis… - Microorganisms, 2020 - mdpi.com
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is essential to limit the spread of the global pandemic we are facing. Quantitative real …