Limits of detection of 6 approved RT–PCR kits for the novel SARS-Coronavirus-2 (SARS-CoV-2)

X Wang, H Yao, X Xu, P Zhang, M Zhang… - Clinical …, 2020 - academic.oup.com
The novel SARS-coronavirus 2 (SARS-CoV-2, previously 2019-nCoV) can cause lethal
coronavirus disease 2019 (COVID-19)(1). Since its outbreak in December 2019, COVID-19 …

Validation of real-time RT-PCR for detection of SARS-CoV-2 in the early stages of the COVID-19 outbreak in the Republic of Korea

YS Chung, NJ Lee, SH Woo, JM Kim, HM Kim, HJ Jo… - Scientific Reports, 2021 - nature.com
A real-time reverse transcription polymerase chain reaction (RT-qPCR) assay that does not
require Emergency Use Authorization (EUA) reagents was tested and validated for the …

An updated review of SARS‐CoV‐2 detection methods in the context of a novel coronavirus pandemic

Y Zhang, Z Huang, J Zhu, C Li, Z Fang… - Bioengineering & …, 2023 - Wiley Online Library
Abstract The World Health Organization has reported approximately 430 million confirmed
cases of coronavirus disease 2019 (COVID‐19), caused by severe acute respiratory …

Predictors of negative first SARS-CoV-2 RT-PCR despite final diagnosis of COVID-19 and association with outcome

JB Lascarrou, G Colin, A Le Thuaut, N Serck… - Scientific Reports, 2021 - nature.com
Reverse transcriptase-polymerase chain reaction (RT-PCR) testing is an important tool for
diagnosing coronavirus disease 2019 (COVID-19). However, performance concerns have …

Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories

T Ishige, S Murata, T Taniguchi, A Miyabe… - Clinica Chimica …, 2020 - Elsevier
Background The detection of SARS-CoV-2 RNA by real-time reverse transcription–
polymerase chain reaction (rRT-PCR) is used to confirm the clinical diagnosis of COVID-19 …

[HTML][HTML] In vitro diagnostics of coronavirus disease 2019: Technologies and application

CC Lai, CY Wang, WC Ko, PR Hsueh - Journal of Microbiology …, 2021 - Elsevier
Laboratory-based diagnostic measures including virological and serological tests are
essential for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …

Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome …

YL Lau, IB Ismail, NIB Mustapa, MY Lai, TS Tuan Soh… - PLoS …, 2021 - journals.plos.org
Rapid diagnosis is an important intervention in managing the Severe Acute Respiratory
Syndrome Coronavirus 2 (SARS-CoV-2) outbreak. Real time reverse transcription …

Comparison of two high-throughput reverse transcription-PCR systems for the detection of severe acute respiratory syndrome coronavirus 2

AR Craney, PD Velu, MJ Satlin… - Journal of Clinical …, 2020 - Am Soc Microbiol
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as the cause
of a worldwide pandemic. Many commercial SARS-CoV-2 reverse transcription-PCR (RT …