Retroviral vectors are still the vectors that are used in the majority of gene therapy trials for
treatment of acquired or inherited diseases. In this review, the present state‐of‐the‐art of the …

Genetically modified cells of haematopoietic and lymphocytic lineages could provide
potentially curative treatments for a wide range of inherited and acquired diseases …

To date, only adherent cell lines have been used for the generation of packaging cells for
the production of type C retrovirus vectors. The large-scale production of high titre retrovirus …

The use of retroviral vectors for human gene therapy requires the production of large
quantities of high titer vector stocks. Maintaining high titers during the prolonged culture of …

Abstract Background An Erratum has been published for this article in Journal of Gene
Medicine 7 (6), 2005, 835. A stable packaging cell line facilitates large‐scale lentivirus …

Retroviral vectors derived from the Moloney murine leukemia virus have been used in
successful and promising gene therapy clinical trials. However, platforms for their large …

Efficient retroviral gene transfer into primary cells is a prerequisite for various gene
therapeutic strategies. We have developed a transduction protocol based on the preloading …

Recombinant retroviruses are now an established tool for gene delivery. Presently they are
mainly produced using adherent cells. However, due to the restrictive nature of adherent cell …

Retroviral vectors have become an important tool for gene transfer in vitro and in vivo.
Classical Moloney murine leukemia virus (MLV) based retroviral vectors have been used for …

Pseudotype vectors are promising for gene transfer in many gene therapy approaches,
however, low-vector concentration in batch cultures and high temperature-dependent decay …