Endogenous retroviruses have developed efficient methods during their life cycle for stable
integration into the host genome. Because of this ability, retroviral vectors were designed …

Retroviral infection or calcium phosphate-mediated DNA transfection has been used for the
generation of retrovirus producing cell lines through the introduction of vector DNA into the …

Self-inactivating lentiviral vectors (LVVs) are used regularly for genetic modification of cells,
including T cells and hematopoietic stem cells for cellular gene therapy. As vector demand …

Background The use of gene modified T lymphocytes for immunotherapy in a cancer or
AIDS clinical trial requires an efficient, safe ex vivo method for modification of these cells at …

Over the last two decades, several attempts to generate packaging cells for lentiviral vectors
(LV) have been made. Despite different technologies, no packaging clone is currently …

Background Lentiviral vectors have emerged as efficient vehicles for transgene delivery in
both dividing and non-dividing cells. A number of different modifications in vector design …

Background During the past twelve years, lentiviral (LV) vectors have emerged as valuable
tools for transgene delivery because of their ability to transduce nondividing cells and their …

Summary Haematopoietic Stem Cells (HSCs) are attractive targets for the gene therapy.
Upon ex vivo gene transfer and transplant, they may generate a progeny of gene-corrected …

Lentiviral vectors are useful experimental tools for stable gene delivery and have been used
to treat human inherited genetic disorders and hematologic malignancies with promising …

Retroviruses are one of the most commonly used vectors in ongoing gene therapy clinical
trials. To evaluate and advance virus production on the microscale platform, we have …