The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding
industry by allowing for the introduction of desirable traits. Although an efficient and targeted …

Humans are sorely over-dependent on livestock for their daily basic need of food in the form
of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the …

Accelerated development of novel CRISPR/Cas9-based genome editing techniques
provides a feasible approach to introduce a variety of precise modifications in the …

Objective Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into
zygotes are used for generating genetically modified pigs. However, these methods create …

Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related
mRNA components into the porcine zygote has been described. Given the relatively long …

Isoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle
caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed …

Background The simplicity of the CRISPR/Cas9 system has enabled its widespread
applications in generating animal models, functional genomic screening and in treating …

The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in
laboratory animals such as mice, rats, zebrafish and pigs. Here, we report that …

Recent advances in the study of the CRISPR/Cas9 system have provided a precise and
versatile approach for genome editing in various species. However, the applicability and …

Myostatin (MSTN), a protein encoded by growth differentiation factor 8 (GDF8), is primarily
expressed in skeletal muscle and negatively regulates the development and regeneration of …