The rare N-unsubstituted glucosamine (GlcN H 3+) residues in heparan sulfate (HS) have
important biological and pathophysiological roles. Because of their low natural abundance …

The separation and quantification of glycosaminoglycan (GAG) chains with different levels of
sulfation from cells and media, and prepared through chemoenzymatic synthesis or …

Introduction: Structural characterization of low molecular weight heparin (LMWH) is critical to
meet biosimilarity standards. In this context, the review focuses on structural analysis of …

Previously, the FDA validated a method to assess the structure and composition of heparin
products by separating and quantifying disaccharide level digests by reverse-phase-ion …

The recently proposed calibrant LHN‐1 (lot F537; henceforth designated F537), for the
molecular weight (MW) determination by high‐performance size‐exclusion chromatography …

Glycosaminoglycans (GAGs) are heterogeneous biomacromolecules made by all animal
cells with overlapping molecular weight and high negative charge densities, which make …

Heparin is a significant anticoagulant that has been used in clinic over decades. Although
numerous efforts have been made to characterize the molecular structure of heparin and its …

We evaluated polyacrylamide gel electrophoresis (PAGE) and size exclusion
chromatography coupled with multi-angle laser light scattering (SEC-MALLS) approaches to …

Heparin is a complex mixture of heterogeneous sulfated polysaccharidic chains. Its physico-
chemical characterization is based on the contribution of several methods, but advantages …

Currently, detailed structural characterization of low-molecular-weight heparin (LMWH)
products is an analytical subject of great interest. In this work, we carried out a …