ABSTRACT CRISPR/Cas‐derived RNA‐guided nucleases (RGNs) that can generate DNA
double‐strand breaks (DSBs) at a specific sequence are widely used for targeted genome …

The rice blast fungus Magnaporthe oryzae is the most serious pathogen of cultivated rice
and a significant threat to global food security. To accelerate targeted mutation and specific …

DNA double‐strand break (DSB) repair induced by the RNA‐programmed nuclease Cas9
has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR …

Precise and straightforward methods to edit the plant genome are much needed for
functional genomics and crop improvement. Recently, RNA-guided genome editing using …

The Streptococcus pyogenes CRISPR-Cas9 system effectively mediates RNA-guided DNA
double-strand breaks and is used for genome editing in many different organisms, including …

Generating mutants bearing multiple gene modifications is essential for 1 determining the
functions of gene family members with redundant functions, or for 2 analyzing epistatic …

Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a
powerful genome-editing method that facilitates genetic alterations of genomes in a variety …

CRISPR/Cas9 uses a guide RNA (gRNA) molecule to execute sequence‐specific DNA
cleavage and it has been widely used for genome editing in many organisms. Modifications …

Genome editing of model organisms is essential for gene function analysis and is thus
critical for human health and agricultural production. The current technologies used for …

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR
associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 …