Strain breeding and gene complementation strategies for filamentous fungi

PA Wang, JJ Zhong - Process Biochemistry, 2023 - Elsevier
Filamentous fungi produce a variety of unique bioactive metabolites, which are important to
food, medical and agricultural industries. In order to better utilize filamentous fungi for …

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

C Woodcraft, YH Chooi, I Roux - Natural Product Reports, 2023 - pubs.rsc.org
Covering: up to May 2022 Fungal genetics has transformed natural product research by
enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced …

CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

M Ullah, L Xia, S Xie, S Sun - Biotechnology and Applied …, 2020 - Wiley Online Library
Filamentous fungi have several industrial, environmental, and medical applications.
However, they are rarely utilized owing to the limited availability of full‐genome sequences …

Strategies for gene disruption and expression in filamentous fungi

YZ Mei, YL Zhu, PW Huang, Q Yang, CC Dai - Applied microbiology and …, 2019 - Springer
Filamentous fungi can produce many valuable secondary metabolites; among these fungi,
endophytic fungi play an ecological role in mutualistic symbiosis with plants, including …

CRISPR-Cas9 system for functional genomics of filamentous fungi: applications and challenges

A Kumar, S Chadha, D Rath - Fungi bio-prospects in sustainable …, 2021 - Elsevier
Functional genomics enhances the understanding of fundamental fungal biology and aids in
improving the production of valuable bioactive fungal compounds. Available gene …

[HTML][HTML] Genetic Engineering of Filamentous Fungi: Prospects for Obtaining Fourth-Generation Biological Products

LR Oliveira, AR Gonçalves, ED Quintela… - Applied …, 2024 - mdpi.com
Filamentous fungi exhibit unparalleled potential as cell factories for protein production,
owing to their adeptness in protein secretion and remarkable proficiency in post …

Progress of the CRISPR/Cas system and the next-generation CRISPR technologies in gene editing for filamentous fungi

C Yingying, LIU Yang, SHI Junjie… - Synthetic Biology …, 2024 - synbioj.cip.com.cn
Filamentous fungi, which harbor distinct morphology and cell structure, play a critical role in
human health and industrial/agricultural production. However, the unique characteristics of …

[HTML][HTML] The CRISPR/Cas9 system for targeted genome engineering in free-living fungi: advances and opportunities for lichenized fungi

K Shanmugam, S Ramalingam… - Frontiers in …, 2019 - frontiersin.org
Studies using whole genome sequencing, computational and gene expression, targeted
genome engineering techniques for generating site-specific sequence alterations through …

[HTML][HTML] CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective

R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

[HTML][HTML] Applications of CRISPR/Cas9 in the synthesis of secondary metabolites in filamentous fungi

C Jiang, G Lv, Y Tu, X Cheng, Y Duan… - Frontiers in …, 2021 - frontiersin.org
Filamentous fungi possess the capacity to produce a wide array of secondary metabolites
with diverse biological activities and structures, such as lovastatin and swainsonine. With the …