[HTML][HTML] CRISPR-Cas9 genome editing approaches in filamentous fungi and oomycetes

M Schuster, R Kahmann - Fungal Genetics and Biology, 2019 - Elsevier
Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

Single crossover-mediated targeted nucleotide substitution and knock-in strategies with CRISPR/Cas9 system in the rice blast fungus

T Yamato, A Handa, T Arazoe, M Kuroki, A Nozaka… - Scientific reports, 2019 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9)-mediated genome editing has become a promising approach for efficient …

Fungal genome editing using CRISPR-Cas nucleases: A new tool for the management of plant diseases

MR Javed, A Ijaz, M Shahid, H Nadeem… - CRISPR and RNAi …, 2021 - Elsevier
Fungal diseases have drastic effects on the quality and yield of crops, thereby developing an
alarming situation for global food security. Filamentous fungi have been the leading …

Development of the CRISPR-Cas9 System for the Marine-Derived Fungi Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7

Y Chen, C Cai, J Yang, J Shi, Y Song, D Hu, J Ma, J Ju - Journal of Fungi, 2022 - mdpi.com
Marine-derived fungi are emerging as attractive producers of structurally novel secondary
metabolites with diverse bioactivities. However, the lack of efficient genetic tools limits the …

CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi

I Roux, C Woodcraft, J Hu, R Wolters… - ACS synthetic …, 2020 - ACS Publications
Accessing the full biosynthetic potential encoded in the genomes of fungi is limited by the
low expression of most biosynthetic gene clusters (BGCs) under common laboratory culture …

Gene editing in filamentous fungi and oomycetes using CRISPR-Cas technology

SK Paul, T Akter, T Islam - CRISPR and RNAi Systems, 2021 - Elsevier
CRISPR toolkit seems most efficient, convenient, cost-effective, and precise for gene editing
in various filamentous fungi. The application of CRISPR technology has been successful in …

Foreshadowing an exponential market: A brief story, applications, and perspectives of CRISPR/Cas system for the production of (hemi-) cellulases in filamentous fungi

FF Silva, NG Rabelo, KC Reis, CSS Cassiano… - Fungal Biology …, 2024 - Elsevier
The demand to develop protein production systems that are both economically and
scientifically viable is reflected in the global scenario, where filamentous fungi, due to their …

Gene editing in dimorphic fungi using CRISPR/Cas9

GC Kujoth, TD Sullivan, BS Klein - Current protocols in …, 2020 - Wiley Online Library
Dimorphic fungi in the genera Blastomyces, Histoplasma, Coccidioides, and
Paracoccidioides are important human pathogens that affect human health in many …

Genome Editing Technology and Its Application Potentials in the Industrial Filamentous Fungus Aspergillus oryzae

J Maruyama - Journal of Fungi, 2021 - mdpi.com
Aspergillus oryzae is a filamentous fungus that has been used in traditional Japanese
brewing industries, such as the sake, soy sauce, and miso production. In addition, A. oryzae …

CRISPR-Cas9 system for fungi genome engineering toward industrial applications

L Satish, S Shamili, BC Muthubharathi… - … Engineering via CRISPR …, 2020 - Elsevier
The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) and
CRISPR associated protein 9 (Cas9) system has emerged as a prominent system for …