Employment of the CRISPR/Cas9 system to improve cellulase production in Trichoderma reesei

S Pant, P Nag, A Ghati, D Chakraborty… - Biotechnology …, 2022 - Elsevier
Trichoderma reesei has been explored intensively in the laboratory and on an industrial
scale for its highly potent cellulase secretion machinery since its characterization over 70 …

Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi

YJ Yang, Y Liu, DD Liu, WZ Guo, LX Wang… - Applied Microbiology …, 2022 - Springer
Recent technical advances regarding filamentous fungi have accelerated the engineering of
fungal-based production and benefited basic science. However, challenges still remain and …

[HTML][HTML] Edible and medicinal fungi breeding techniques, a review: Current status and future prospects

Y Dong, R Miao, R Feng, T Wang, J Yan, X Zhao… - Current Research in …, 2022 - Elsevier
Mushrooms of the edible and medicinal which are highly nutritious and environmentally
friendly crops carry numerous medicinal benefits. For the abundant and high diversity of …

CRISPR/Cas-based genome editing in Aspergillus niger

X Zheng, P Zheng, J Sun - Sheng wu Gong Cheng xue bao …, 2021 - europepmc.org
Aspergillus niger is a vital industrial workhouse widely used for the production of organic
acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance …

Targeted genome editing using CRISPR/Cas9 system in fungi

T Arazoe, O Mizutani - Genome Engineering via CRISPR-Cas9 System, 2020 - Elsevier
Fungi are eukaryotic microbes, comprising species that are involved in various food,
agricultural, and pharmaceutical industries. Some fungal species are pathogenic to humans …

Application of CRISPR/Cas9 editing for production of secondary metabolites in Actinomycetes

JA Bai, RV Rai - … Products from Actinomycetes: Diversity, Ecology and …, 2022 - Springer
The CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9-based RNA-
guided DNA editing technique is used to make double-stranded breaks in genomes. Further …

CRISPR/Cas9 facilitates rapid generation of constitutive forms of transcription factors in Aspergillus niger through specific on-site genomic mutations resulting in …

RS Kun, J Meng, S Salazar-Cerezo, MR Mäkelä… - Enzyme and microbial …, 2020 - Elsevier
The CRISPR/Cas9 system has been successfully applied for gene editing in filamentous
fungi. Previous studies reported that single stranded oligonucleotides can be used as repair …

Efficient genome editing in Fusarium oxysporum based on CRISPR/Cas9 ribonucleoprotein complexes

Q Wang, PA Cobine, JJ Coleman - Fungal Genetics and Biology, 2018 - Elsevier
The Fusarium oxysporum species complex (FOSC) is an economically important group of
pathogenic filamentous fungi that are able to infect both animals and plants. Reverse …

Tailoring in fungi for next generation cellulase production with special reference to CRISPR/CAS system

S Mondal, SK Halder, KC Mondal - Systems Microbiology and …, 2022 - Springer
Cellulose is the utmost plenteous source of biopolymer in our earth, and fungi are the most
efficient and ubiquitous organism in degrading the cellulosic biomass by synthesizing …

Efficient gene editing in Neurospora crassa with CRISPR technology

T Matsu-Ura, M Baek, J Kwon, C Hong - Fungal biology and biotechnology, 2015 - Springer
Background Efficient gene editing is a critical tool for investigating molecular mechanisms of
cellular processes and engineering organisms for numerous purposes ranging from …