DNA double‐strand break (DSB) repair induced by the RNA‐programmed nuclease Cas9
has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR …

Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …

Abstract Background The CRISPR/Cas9 is currently the predominant technology to enhance
the genome editing efficiency in eukaryotes. Established tools for many fungal species exist …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

Filamentous fungi represent an invaluable source of pharmaceutically active compounds.
The development of versatile methods to genetically manipulate filamentous fungi is of great …

In recent years, a variety of genetic tools have been developed and applied to various
filamentous fungi, which are widely applied in agriculture and the food industry. However …

Objectives To develop a genome editing method using the CRISPR/Cas9 system in
Aspergillus oryzae, the industrial filamentous fungus used in Japanese traditional …

Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a
powerful genome-editing method that facilitates genetic alterations of genomes in a variety …

ABSTRACT CRISPR/Cas‐derived RNA‐guided nucleases (RGNs) that can generate DNA
double‐strand breaks (DSBs) at a specific sequence are widely used for targeted genome …

Background Within the last years, numerous reports described successful application of the
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …