Aiming to increase the reproducibility of biomedical research results, biobanks obtain
human tissues of the highest quality and carry out different storage methods adapted to the …

Background Functional 3D organ models such as precision-cut lung slices (PCLS) have
recently captured the attention of biomedical research. To enable wider implementation in …

Lung cancer is the most common cause of cancer-related deaths worldwide, and metastatic
spread of the cancer rather than the primary tumor is the main cause of death. However, the …

Analysis of RNA isolated from fixed and paraffin-embedded tissues is widely used in
biomedical research and molecular pathological diagnostics. We have performed a …

In recent years the improvements in high-throughput gene expression analysis have led to
the discovery of numerous non-protein-coding RNA (npcRNA) molecules. They form an …

Background RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is
chemically modified and degraded, which compromises its use in gene expression studies …

The accuracy of information garnered by real-time quantitative polymerase chain reaction
(RT-qPCR), an important technology for elucidating molecular mechanisms of disease, is …

Abstract Analysis of multiple analytes from biological samples can be challenging as
different analytes require different preservation measures. Heat induced enzymatic …

The quality of tissue studied impacts greatly on oligonucleotide microarray results,
emphasizing the importance of harvesting techniques. The analyzed RNA extracted from …

Real-time reverse transcription polymerase chain reaction (PCR) is the gold standard for
expression analysis. Designed to improve reproducibility and sensitivity, commercial kits are …