GONAD: A new method for germline genome editing in mice and rats
M Namba, T Kobayashi, T Koyano… - Development …, 2021 - Wiley Online Library
Recent advances in the CRISPR/Cas9 system have demonstrated it to be an efficient gene‐
editing technology for various organisms. Laboratory mice and rats are widely used as …
editing technology for various organisms. Laboratory mice and rats are widely used as …
GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos
CB Gurumurthy, G Takahashi, K Wada… - Current protocols in …, 2016 - Wiley Online Library
Transgenic technologies used for creating a desired genomic change in animals involve
three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them …
three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them …
[HTML][HTML] Successful production of genome-edited rats by the rGONAD method
T Kobayashi, M Namba, T Koyano, M Fukushima… - BMC …, 2018 - Springer
Background Recent progress in development of the CRISPR/Cas9 system has been shown
to be an efficient gene-editing technology in various organisms. We recently developed a …
to be an efficient gene-editing technology in various organisms. We recently developed a …
[图书][B] Recent Advances in In Vivo Genome Editing Targeting Mammalian Preimplantation Embryos
M Sato, M Ohtsuka, E Inada, S Nakamura, I Saitoh… - 2022 - intechopen.com
CRISPR-based genome engineering has been widely used for producing gene-modified
animals such as mice and rats, to explore the function of a gene of interest and to create …
animals such as mice and rats, to explore the function of a gene of interest and to create …
Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
CB Gurumurthy, M Sato, A Nakamura, M Inui… - Nature protocols, 2019 - nature.com
Methods to create genetically engineered mice involve three major steps: harvesting
embryos from one set of females, microinjection of reagents into embryos ex vivo and their …
embryos from one set of females, microinjection of reagents into embryos ex vivo and their …
[HTML][HTML] Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice
M Sato, R Miyagasako, S Takabayashi, M Ohtsuka… - Cells, 2020 - mdpi.com
Improved genome-editing via oviductal nucleic acid delivery (i-GONAD) is a technique
capable of inducing genomic changes in preimplantation embryos (zygotes) present within …
capable of inducing genomic changes in preimplantation embryos (zygotes) present within …
[HTML][HTML] A non-inheritable maternal Cas9-based multiple-gene editing system in mice
T Sakurai, A Kamiyoshi, H Kawate, C Mori… - Scientific reports, 2016 - nature.com
The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote
injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or …
injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or …
Simplified pipelines for genetic engineering of mammalian embryos by CRISPR-Cas9 electroporation
Gene editing technologies, such as CRISPR-Cas9, have important applications in
mammalian embryos for generating novel animal models in biomedical research and lines …
mammalian embryos for generating novel animal models in biomedical research and lines …
Concepts and tools for gene editing
S Josa, D Seruggia, A Fernández… - Reproduction, Fertility …, 2017 - CSIRO Publishing
Gene editing is a relatively recent concept in the molecular biology field. Traditional genetic
modifications in animals relied on a classical toolbox that, aside from some technical …
modifications in animals relied on a classical toolbox that, aside from some technical …
[HTML][HTML] i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases
M Ohtsuka, M Sato, H Miura, S Takabayashi… - Genome biology, 2018 - Springer
We present a robust method called improved-Genome editing via Oviductal Nucleic Acids
Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0. 7 embryos via in situ …
Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0. 7 embryos via in situ …