Recent advances in the CRISPR/Cas9 system have demonstrated it to be an efficient gene‐
editing technology for various organisms. Laboratory mice and rats are widely used as …

Transgenic technologies used for creating a desired genomic change in animals involve
three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them …

Background Recent progress in development of the CRISPR/Cas9 system has been shown
to be an efficient gene-editing technology in various organisms. We recently developed a …

CRISPR-based genome engineering has been widely used for producing gene-modified
animals such as mice and rats, to explore the function of a gene of interest and to create …

Methods to create genetically engineered mice involve three major steps: harvesting
embryos from one set of females, microinjection of reagents into embryos ex vivo and their …

Improved genome-editing via oviductal nucleic acid delivery (i-GONAD) is a technique
capable of inducing genomic changes in preimplantation embryos (zygotes) present within …

The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote
injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or …

Gene editing technologies, such as CRISPR-Cas9, have important applications in
mammalian embryos for generating novel animal models in biomedical research and lines …

Gene editing is a relatively recent concept in the molecular biology field. Traditional genetic
modifications in animals relied on a classical toolbox that, aside from some technical …

We present a robust method called improved-Genome editing via Oviductal Nucleic Acids
Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0. 7 embryos via in situ …