Superfolder GFP reporters validate diverse new mRNA targets of the classic porin regulator, MicF RNA

CP Corcoran, D Podkaminski, K Papenfort… - Molecular …, 2012 - Wiley Online Library
MicF is a textbook example of a small regulatory RNA (sRNA) that acts on a trans‐encoded
target mRNA through imperfect base pairing. Discovery of MicF as a post‐transcriptional …

[HTML][HTML] Global RNA interactome of Salmonella discovers a 5′ UTR sponge for the MicF small RNA that connects membrane permeability to transport capacity

G Matera, Y Altuvia, M Gerovac, Y El Mouali, H Margalit… - Molecular cell, 2022 - cell.com
The envelope of Gram-negative bacteria is a vital barrier that must balance protection and
nutrient uptake. Small RNAs are crucial regulators of the envelope composition and function …

A role for the Escherichia coli H‐NS‐like protein StpA in OmpF porin expression through modulation of micF RNA stability

P Deighan, A Free, CJ Dorman - Molecular Microbiology, 2000 - Wiley Online Library
When a wild‐type strain of Escherichia coli and its stpA, hns and stpA hns mutant derivatives
were compared by two‐dimensional protein gel electrophoresis, the levels of expression of …

MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors

N Delihas, S Forst - Journal of molecular biology, 2001 - Elsevier
The micF gene is a stress response gene found in Escherichia coli and related bacteria that
post-transcriptionally controls expression of the outer membrane porin gene ompF. The micF …

Systematic deletion of Salmonella small RNA genes identifies CyaR, a conserved CRP‐dependent riboregulator of OmpX synthesis

K Papenfort, V Pfeiffer, S Lucchini… - Molecular …, 2008 - Wiley Online Library
Post‐transcriptional repression of porin synthesis has emerged as a major function of Hfq‐
dependent, small non‐coding RNAs (sRNAs). Many enterobacteria express OmpX‐like …

Recognition of heptameric seed sequence underlies multi‐target regulation by RybB small RNA in Salmonella enterica

R Balbontín, F Fiorini, N Figueroa‐Bossi… - Molecular …, 2010 - Wiley Online Library
Prokaryotic regulatory small RNAs act by a conserved mechanism and yet display a
stunning structural variability. In the present study, we used mutational analysis to dissect …

A mixed double negative feedback loop between the sRNA MicF and the global regulator Lrp

E Holmqvist, C Unoson, J Reimegård… - Molecular …, 2012 - Wiley Online Library
Roughly 10% of all genes in Escherichia coli are controlled by the global transcription factor
Lrp, which responds to nutrient availability. Bioinformatically, we identified lrp as one of …

[HTML][HTML] Discovery and characterization of the first non-coding RNA that regulates gene expression, micF RNA: A historical perspective

N Delihas - World journal of biological chemistry, 2015 - ncbi.nlm.nih.gov
The first evidence that RNA can function as a regulator of gene expression came from
experiments with prokaryotes in the 1980s. It was shown that Escherichia coli micF is an …

In vivo RNA interactome profiling reveals 3'UTR-processed small RNA targeting a central regulatory hub

F Liu, Z Chen, S Zhang, K Wu, C Bei, C Wang… - Nature …, 2023 - nature.com
Small noncoding RNAs (sRNAs) are crucial regulators of gene expression in bacteria.
Acting in concert with major RNA chaperones such as Hfq or ProQ, sRNAs base-pair with …

A conserved RpoS-dependent small RNA controls the synthesis of major porin OmpD

KS Fröhlich, K Papenfort, AA Berger… - Nucleic acids …, 2012 - academic.oup.com
A remarkable feature of many small non-coding RNAs (sRNAs) of Escherichia coli and
Salmonella is their accumulation in the stationary phase of bacterial growth. Several stress …