Zooming in on protein–RNA interactions: a multi-level workflow to identify interaction partners

A Colantoni, J Rupert, A Vandelli… - Biochemical Society …, 2020 - portlandpress.com
Interactions between proteins and RNA are at the base of numerous cellular regulatory and
functional phenomena. The investigation of the biological relevance of non-coding RNAs …

mRNA structure regulates protein expression through changes in functional half-life

DM Mauger, BJ Cabral, V Presnyak… - Proceedings of the …, 2019 - National Acad Sciences
Messenger RNAs (mRNAs) encode information in both their primary sequence and their
higher order structure. The independent contributions of factors like codon usage and …

Universally increased mRNA stability downstream of the translation initiation site in eukaryotes and prokaryotes

Y Mao, W Wang, N Cheng, Q Li, S Tao - Gene, 2013 - Elsevier
Local secondary structures in coding sequences have important functions across various
translational processes. To date, however, the local structures and their functions in the early …

Targeting RNA‐binding proteins with small molecules: Perspectives, pitfalls and bifunctional molecules

Q Li, C Kang - FEBS letters, 2023 - Wiley Online Library
RNA‐binding proteins (RBPs) play vital roles in organisms through binding with RNAs to
regulate their functions. Small molecules affecting the function of RBPs have been …

Optimized RNA gel-shift and UV cross-linking assays for characterization of cytoplasmic RNA-protein interactions

AM Thomson, JT Rogers, CE Walker, JM Staton… - …, 1999 - Taylor & Francis
Considerable interest has recently focused on defining the mechanisms involved in the
regulation of gene expression at the level of mRNA stability and translational efficiency …

Engineering RNA‐binding proteins with diverse activities

H Wei, Z Wang - Wiley Interdisciplinary Reviews: RNA, 2015 - Wiley Online Library
With generations of efforts to understand RNA functions in diverse cellular processes, RNA‐
binding proteins (RBPs) have emerged to be one of the central players in regulating RNA …

Large-scale tethered function assays identify factors that regulate mRNA stability and translation

EC Luo, JL Nathanson, FE Tan, JL Schwartz… - Nature structural & …, 2020 - nature.com
The molecular functions of the majority of RNA-binding proteins (RBPs) remain unclear,
highlighting a major bottleneck to a full understanding of gene expression regulation. Here …

[HTML][HTML] Single-molecule mRNA decay measurements reveal promoter-regulated mRNA stability in yeast

T Trcek, DR Larson, A Moldón, CC Query, RH Singer - Cell, 2011 - cell.com
Messenger RNA decay measurements are typically performed on a population of cells.
However, this approach cannot reveal sufficient complexity to provide information on …

Cooperativity in RNA–protein interactions: the complex is more than the sum of its partners

T Achsel, C Bagni - Current opinion in neurobiology, 2016 - Elsevier
Highlights•Neuropathologies are often linked to mutations in RNA-binding proteins
(RBPs).•RBPs recognise their targets in combination with other RBPs.•Depending on the …

From cis-regulatory elements to complex RNPs and back

F Gebauer, T Preiss… - Cold Spring Harbor …, 2012 - cshperspectives.cshlp.org
Messenger RNAs (mRNAs), the templates for translation, have evolved to harbor abundant
cis-acting sequences that affect their posttranscriptional fates. These elements are frequently …