Reliability of real-time RT-PCR tests to detect SARS-Cov-2: A literature review

C Bezier, G Anthoine, A Charki - International Journal of …, 2020 - metrology-journal.org
In the face of the COVID-19 (Coronavirus Disease 2019) pandemic, the World Health
Organization (WHO) has urged countries to test the population more widely. Clinical …

Analysis of external quality assessment samples revealed crucial performance differences between commercial RT-PCR assays for SARS-CoV-2 detection when …

M Malecki, J Luesebrink, AF Wendel… - Journal of Virological …, 2021 - Elsevier
In limelight of the ongoing pandemic SARS-CoV-2 testing is critical for the diagnosis of
infected patients, contact-tracing and mitigating the transmission. Diagnostic laboratories are …

[PDF][PDF] External peer review of the RTPCR test to detect SARS-CoV-2 reveals 10 major scientific flaws at the molecular and methodological level: consequences for …

P Borger, BR Malhotra, M Yeadon, C Craig… - Preprint …, 2020 - howbadismybatch.com
In the publication entitled “Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-
PCR”(Eurosurveillance 25 (8) 2020) the authors present a diagnostic workflow and RT …

Comparison and sensitivity evaluation of three different commercial real-time quantitative PCR kits for SARS-CoV-2 detection

A Banko, G Petrovic, D Miljanovic, A Loncar… - Viruses, 2021 - mdpi.com
Real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most sensitive
and specific assay and, therefore, is the “gold standard” diagnostic method for the diagnosis …

Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative

Y Zhou, F Pei, M Ji, L Wang, H Zhao, H Li, W Yang… - PloS one, 2020 - journals.plos.org
The early detection and differential diagnosis of respiratory infections increase the chances
for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the …

Analytical performance of COVID-19 detection methods (RT-PCR): scientific and societal concerns

R Verna, W Alallon, M Murakami, CPM Hayward… - Life, 2021 - mdpi.com
Background. Health and social management of the SARS-CoV-2 epidemic, responsible for
the COVID-19 disease, requires both screening tools and diagnostic procedures. Reliable …

[HTML][HTML] A comparative study of real-time RT-PCR–based SARS-CoV-2 detection methods and its application to human-derived and surface swabbed material

A Tastanova, CI Stoffel, A Dzung, PF Cheng… - The Journal of Molecular …, 2021 - Elsevier
Real-time RT-PCR remains a gold standard in the detection of various viral diseases. In the
coronavirus 2019 pandemic, multiple RT-PCR–based tests were developed to screen for …

Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2

Y Wu, W Xu, Z Zhu, X Xia - Journal of Clinical Laboratory …, 2020 - Wiley Online Library
Background Reverse transcription‐polymerase chain reaction (RT‐PCR) is an extremely
common clinical method for detecting pathogens, particularly for emerging infectious …

[PDF][PDF] Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives

KS Liu, XD Mao, W Ni, TP Li - Heliyon, 2022 - cell.com
Abstract Nowadays, coronavirus disease 2019 (COVID-19) caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2), whose infectivity is awfully strong, has …

rRT-PCR for SARS-CoV-2: Analytical considerations

R Rahbari, N Moradi, M Abdi - Clinica Chimica Acta, 2021 - Elsevier
The COVID-19 pandemic remains a significant problem involving health systems worldwide.
Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS …