Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

LB Ferré, ME Kjelland, AM Taiyeb… - … in Domestic Animals, 2020 - Wiley Online Library
Contents Cryopreservation of in vitro‐derived bovine embryos is a crucial step for the
widespread reproduction and conservation of valuable high‐merit animals. Given the …

Basic and applied features in the cryopreservation progress of bovine embryos

RS Valente, TV Marsico, MJ Sudano - Animal Reproduction Science, 2022 - Elsevier
The expansion of the use of in vitro production techniques has revolutionized the bovine
embryo market. In the last decade, we have seen the number of in vitro produced (IVP) …

Survival of vitrified in vitro–produced bovine embryos after a one-step warming in-straw cryoprotectant dilution procedure

JN Caamaño, E Gómez, B Trigal, M Muñoz… - Theriogenology, 2015 - Elsevier
Vitrification is an alternative to slow-rate freezing for cryopreserving bovine embryos.
However, this technology requires simplification if it is to be used under field conditions. The …

Cryopreservation of ova and embryos from livestock: current status and research needs

H Niemann - Theriogenology, 1991 - Elsevier
Over the last approximately 17 years freezing procedures have been developed allowing
high survival rates for nonsurgically collected bovine morulae and blastocysts. The vast …

Update on the vitrification of bovine oocytes and in vitro-produced embryos

T Mogas - Reproduction, Fertility and Development, 2019 - CSIRO Publishing
The combined use of reproductive technologies, such as transvaginal ovum-pick up and in
vitro embryo production followed by direct transfer of cryopreserved embryos, has great …

Transfer of fresh and cryopreserved IVP bovine embryos: normal calving, birth weight and gestation lengths

Y Agca, RL Monson, DL Northey, OA Mazni… - Theriogenology, 1998 - Elsevier
In vitro and in vivo developmental competence of fresh and cryopreserved in vitro produced
(IVP) bovine embryos was evaluated up to birth. Three experiments were done. The …

Cryopreservation of bovinein VitroProduced embryos using ethylene glycol in controlled freezing or vitrification

V Sommerfeld, H Niemann - Cryobiology, 1999 - Elsevier
In this study, the cryoprotectant ethylene glycol (EG) was tested for its ability to improve and
facilitate the cryopreservation ofin vitroproduced (IVP) bovine embryos. Embryos were …

In-straw cryoprotectant dilution for bovine embryos vitrified using Cryotop

Y Inaba, Y Aikawa, T Hirai, Y Hashiyada… - … of Reproduction and …, 2011 - jstage.jst.go.jp
The aim of this study was to develop an in-straw dilution method suitable for 1-step bovine
embryo transfer of vitrified embryos using the Cryotop vitrification-straw dilution (CVSD) …

Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium

E Gómez, S Carrocera, D Martín, JJ Pérez-Jánez… - Theriogenology, 2020 - Elsevier
Direct transfer (DT) of cryopreserved embryos to recipients facilitates on-farm application.
We analyzed a new freezing/thawing (F/T) procedure for in vitro produced (IVP) embryos …

Field trial to compare pregnancy rates of bovine embryo cryopreservation methods: vitrification and one-step dilution versus slow freezing and three-step dilution

AM Van Wagtendonk-de Leeuw, JHG Den Daas… - Theriogenology, 1997 - Elsevier
We designed and conducted a field trial to obtain accurate pregnancy rates of Day 7 bovine
embryos after vitrification in PB1 containing 6.5 M glycerol and 6% BSA (w/v) and one-step …