Single-vesicle assays using liposomes and cell-derived vesicles: from modeling complex membrane processes to synthetic biology and biomedical applications

H Pick, AC Alves, H Vogel - Chemical reviews, 2018 - ACS Publications
The plasma membrane is of central importance for defining the closed volume of cells in
contradistinction to the extracellular environment. The plasma membrane not only serves as …

Advancement and obstacles in microfluidics-based isolation of extracellular vesicles

M Havers, A Broman, A Lenshof, T Laurell - Analytical and Bioanalytical …, 2023 - Springer
There is a great need for techniques which enable reproducible separation of extracellular
vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of …

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

J Kowal, G Arras, M Colombo… - Proceedings of the …, 2016 - National Acad Sciences
Extracellular vesicles (EVs) have become the focus of rising interest because of their
numerous functions in physiology and pathology. Cells release heterogeneous vesicles of …

Ultrasensitive single extracellular vesicle detection using high throughput droplet digital enzyme-linked immunosorbent assay

Z Yang, Y Atiyas, H Shen, MJ Siedlik, J Wu, K Beard… - Nano …, 2022 - ACS Publications
Extracellular vesicles (EVs) have attracted enormous attention for their diagnostic and
therapeutic potential. However, it has proven challenging to achieve the sensitivity to detect …

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion …

JA Martínez‐Greene… - Journal of …, 2021 - Wiley Online Library
The molecular characterization of extracellular vesicles (EVs) has revealed a great
heterogeneity in their composition at a cellular and tissue level. Current isolation methods …

Diurnal variations of circulating extracellular vesicles measured by nano flow cytometry

KM Danielson, J Estanislau, J Tigges, V Toxavidis… - PloS one, 2016 - journals.plos.org
The identification of extracellular vesicles (EVs) as intercellular conveyors of biological
information has recently emerged as a novel paradigm in signaling, leading to the …

Extracellular vesicles: translational challenges and opportunities

H Clemmens, DW Lambert - Biochemical Society Transactions, 2018 - portlandpress.com
Extracellular vesicles (EVs) are a heterogeneous group of small lipid-enclosed structures
with myriad roles in physiology and disease. The recent surge of interest in EVs has led to …

Advanced microfluidic technologies for isolating extracellular vesicles

S Zhang, J Deng, J Li, F Tian, C Liu, L Fang… - TrAC Trends in Analytical …, 2022 - Elsevier
Extracellular vesicles (EVs) are lipid membrane-enclosed, cell-secreted nanovesicles that
have attracted significant attentions in recent years in view of their potential as non-invasive …

Framework for rapid comparison of extracellular vesicle isolation methods

D Ter-Ovanesyan, M Norman, R Lazarovits, W Trieu… - Elife, 2021 - elifesciences.org
Extracellular vesicles (EVs) are released by all cells into biofluids and hold great promise as
reservoirs of disease biomarkers. One of the main challenges in studying EVs is a lack of …

Integrated microfluidic device for accurate extracellular vesicle quantification and protein markers analysis directly from human whole blood

S Zhou, T Hu, F Zhang, D Tang, D Li, J Cao… - Analytical …, 2019 - ACS Publications
Extracellular vesicles (EVs) have the potential to be utilized as disease-specific biomarkers.
Although strategies for on-chip isolation and detection of EVs have recently been …